细胞分离机的类型，费森尤斯 Amicus 与弗雷森纽斯科尔特，可能影响供者血液的细胞成分值。

Type of Cell Separator, Fenwal Amicus vs Fresenius Com Tec, May Influence the Corpuscular Elements Value of the Donor`s Blood.

机构信息

Institute of Transfusion Medicine F B&H, Cekalusa 86, Sarajevo, Bosnia and Herzegovina,

出版信息

Psychiatr Danub. 2019 Dec;31(Suppl 5):821-825.

Abstract

INTRODUCTION

During the plateletpheresis procedure the number of thrombocytes in the donor's blood significantly decreases, and the levels of the hematocrit (HCT), hemoglobin (Hgb), and leukocyte (WBC) diminish as well. Influence of the cell separator is one of the factors that affects the levels of HCT, Hgb and WBC. In this study, the goal was to determine the value difference of HCT, Hgb, WBC, and platelets after the platelet pheresis process between performance on Fenwal AMICUS and on Fresenius Com Tec.

DONORS AND METHODS

The criteria for participation: male in the age range of 25-45. We have formed two groups: for both groups - 180 separations were performed on 60 participants were the values of hematocrits, concentration of hemoglobin and number of leukocytes were established before and after separation using the double-needle continuous flow cell separation (DN-CFCS) on two different devices, Fenwal AMICUS device and the Fresenius Com Tec. device. To confirm the statistical differences we have used Student t-test for independent or dependent samples, as well as Mann-Whitney U test as non-parametric alternative. To compare differences between the values of four parameters (P1-P2) from two groups (using two devices - Fenwal AMICUS and Fresenius Com Tec) The possibility of errors were accepted for α<0.05, and the difference between groups as statistical relevant were accepted for p<0.05.

RESULTS

Statistically significant lower values were noted for all researched parameters after separation on both devices. The statistically significant average values for Hct, Hgb and WBC obtained between two devices, were less than 0.05 (p=0.05). For the platelets (Plt) there was no statistical significant difference (p>0.05 - α=0.05), between average level obtained using either Fenwal AMICUS or Frazenius Com Tec.

CONCLUSION

The type of cell separator had the influence on the decrease value of the observed parameters.

摘要

简介

在血小板单采过程中，供体血液中的血小板数量显著减少，同时血细胞比容（HCT）、血红蛋白（Hgb）和白细胞（WBC）水平也会降低。细胞分离机的性能是影响 HCT、Hgb 和 WBC 水平的因素之一。在这项研究中，我们的目的是确定在 Fenwal AMICUS 和 Fresenius Com Tec 上进行血小板单采后，HCT、Hgb、WBC 和血小板的差值。

捐赠者和方法

参与标准：年龄在 25-45 岁之间的男性。我们将参与者分为两组：每组 60 人，共进行了 180 次分离。使用双针连续流细胞分离（DN-CFCS）在两种不同的设备（Fenwal AMICUS 设备和 Fresenius Com Tec 设备）上，在分离前后建立了血细胞比容、血红蛋白浓度和白细胞数量的值。为了确认统计学差异，我们使用了独立或依赖样本的学生 t 检验，以及非参数替代的曼-惠特尼 U 检验。为了比较两组（使用两种设备 - Fenwal AMICUS 和 Fresenius Com Tec）的四个参数（P1-P2）值之间的差异，我们接受了α<0.05 的错误可能性，并且接受了 p<0.05 的组间差异作为统计学相关。

结果

两种设备分离后，所有研究参数的值均显著降低。两种设备之间获得的 Hct、Hgb 和 WBC 的平均统计值显著低于 0.05（p=0.05）。对于血小板（Plt），没有统计学上的显著差异（p>0.05 - α=0.05），无论是使用 Fenwal AMICUS 还是 Frazenius Com Tec，获得的平均水平都没有统计学差异。