利用碳点-MnO 探针对海洋食品样品中过氧化氢进行“开启”型荧光传感检测。

'Turn-on' fluorescence sensing of hydrogen peroxide in marine food samples using a carbon dots-MnO probe.

机构信息

School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, China.

Engineering Technology Research Center for Grain & Oil Food, State Administration of Grain, Henan University of Technology, Zhengzhou, China.

出版信息

Luminescence. 2020 Sep;35(6):897-902. doi: 10.1002/bio.3799. Epub 2020 Mar 11.

DOI:10.1002/bio.3799

PMID:32160380

Abstract

A 'turn-on' fluorescence method for detection of hydrogen peroxide (H O ) in marine food samples is presented in this article. Using this method, a carbon dots (CDs)-MnO probe was formed in which fluorescence intensity (FI) of CDs was quenched through fluorescence resonance energy transfer by addition of MnO nanosheets. When H O was added into the CDs-MnO solution, the MnO nanosheets formed Mn ions due to a redox reaction between H O and MnO nanosheets, and CD FI was recovered. Under optimized conditions, the detection limit for H O was 0.87 μM, and analytical linear range was 4-100 μM. Furthermore, this developed fluorescence sensing system was successfully used with satisfactory results to determine trace H O content in marine food samples.

摘要

本文提出了一种用于检测海洋食品样品中过氧化氢（H₂O₂）的“开启”荧光方法。在该方法中，形成了碳点（CDs）-MnO 探针，其中通过添加 MnO 纳米片，通过荧光共振能量转移使 CDs 的荧光强度（FI）猝灭。当 H₂O₂加入到 CDs-MnO 溶液中时，MnO 纳米片由于 H₂O₂和 MnO 纳米片之间的氧化还原反应而形成 Mn 离子，并且 CD FI 得到恢复。在优化条件下，H₂O₂的检测限为 0.87 μM，分析线性范围为 4-100 μM。此外，该开发的荧光传感系统成功地用于海洋食品样品中痕量 H₂O₂含量的测定，结果令人满意。