Small Animal Clinic, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000, Ljubljana, Slovenia.
Institute of Pathology, Wild Animals, Fish and Bees, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000, Ljubljana, Slovenia.
BMC Vet Res. 2020 Feb 24;16(1):87. doi: 10.1186/s12917-020-02290-x.
Glycomer 631 and lactomer 9-1 are absorbable suture materials indicated for soft tissue approximation in non-infected wounds. Pigs are often used as surgical models in translational research; however, reports of tissue reactions to both suture materials in pigs are limited. The aim of this study was to assess clinical and histologic responses of skin incisions closed with a subcuticular technique using glycomer 631 and lactomer 9-1 in pigs.
Skin incisions on 17 pigs were closed with glycomer 631 and lactomer 9-1, and a clinical reactive score (CRS) including erythema, swelling, discharge and dehiscence was calculated on postoperative days 7 and 14. Subcuticular tissue reaction was assessed histologically on postoperative day 14 (the presence of extravascular neutrophils, macrophages, multinucleated giant cells, lymphocytes, fibroblasts, bacterial colonies and the overall severity of the inflammatory response to the suture material), and the cumulative score of the variables was calculated as an aggregate tissue irritation score (ATIS). Tissue samples were examined for suture extrusion and evaluated microbiologically. The clinical reactive score did not differ between the suture materials. Only one ATIS variable, namely the overall severity of the inflammatory response, was lower (p = 0.029) when glycomer 631 was used. Suture extrusion was found in 10/17 of the incisions closed by glycomer 631 and in 7/13 of the incisions closed by lactomer 9-1. Trueperella pyogenes was isolated from the skin and from the area of tissue reaction in six pigs.
No difference in CRS between the suture materials was observed, and thus both materials may be used for the subcuticular technique in pigs. Glycomer 631 induced less tissue reaction only in terms of the overall severity of the inflammatory response. Suture extrusion was observed in more than 50% of incisions regardless of the suture material, possibly due to a large amount of suture material in the wound. Trueperella pyogenes was the only pathogen isolated from the tissue surrounding the suture material.
Glycomer 631 和 lactomer 9-1 是可吸收缝线材料，用于非感染性伤口的软组织接近。猪常用于转化研究中的手术模型；然而，关于这两种缝线材料在猪体内的组织反应的报告有限。本研究旨在评估使用 Glycomer 631 和 lactomer 9-1 进行皮下技术缝合的猪皮肤切口的临床和组织学反应。
17 头猪的皮肤切口用 Glycomer 631 和 lactomer 9-1 缝合，并在术后第 7 天和第 14 天计算包括红斑、肿胀、分泌物和裂开的临床反应评分 (CRS)。术后第 14 天评估皮下组织反应（血管外中性粒细胞、巨噬细胞、多核巨细胞、淋巴细胞、成纤维细胞、细菌菌落的存在以及缝线材料引起的炎症反应的总体严重程度），并计算变量的累积评分作为总组织刺激评分 (ATIS)。检查缝线挤出物并进行微生物学评估组织样本。缝线材料之间的临床反应评分没有差异。只有一个 ATIS 变量，即炎症反应的总体严重程度，当使用 Glycomer 631 时较低（p=0.029）。在 10/17 例用 Glycomer 631 缝合的切口和 13/13 例用 lactomer 9-1 缝合的切口发现缝线挤出。六头猪的皮肤和组织反应区分离出 Trueperella pyogenes。
缝线材料之间未观察到 CRS 差异，因此两种材料均可用于猪的皮下技术。Glycomer 631 仅在炎症反应的总体严重程度方面引起的组织反应较少。无论缝线材料如何，超过 50%的切口都出现缝线挤出，这可能是由于伤口中有大量缝线材料。从缝线材料周围的组织中仅分离出 Trueperella pyogenes 这一种病原体。
