Ierusalimsky Victor N, Roshchin Matvey V, Balaban Pavel M
Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, 5A Butlerova str., Moscow, Russia, 117485.
Cell Mol Neurobiol. 2020 Nov;40(8):1395-1404. doi: 10.1007/s10571-020-00825-2. Epub 2020 Mar 11.
In the present work, using in situ hybridization, we studied the expression patterns of three molluscan homologs of vertebrate immediate-early genes C/EBP, c-Fos, and c-Jun in the central nervous system (CNS) of terrestrial gastropod snail Helix. The molluscan C/EBP gene was described in literature, while c-Fos and c-Jun were studied in terrestrial snails for the first time. Localization of the expression was traced in normal conditions, and in preparations physiologically activated using stimulation of suboesophageal ganglia nerves. No expression was detected constitutively. In stimulated preparations, all three genes had individual expression patterns in Helix CNS, and the level of expression was stimulus-dependent. The number of cells expressing the gene of interest was different from the number of cells projecting to the stimulated nerve, and thus activated retrogradely. This difference depended on the ganglia studied. At the subcellular level, the labeled RNA was observed as dots (probably small clusters of RNA molecules) and shapeless mass of RNA, often seen as a circle at the internal border of the cell nuclei. The data provide a basis for further study of behavioral role of these putative immediate-early genes in snail behavior and learning.
在本研究中，我们利用原位杂交技术，研究了陆生腹足纲蜗牛Helix中枢神经系统（CNS）中脊椎动物即刻早期基因C/EBP、c-Fos和c-Jun的三个软体动物同源基因的表达模式。软体动物的C/EBP基因已有文献报道，而c-Fos和c-Jun是首次在陆生蜗牛中进行研究。在正常条件下以及在通过刺激食管下神经节神经进行生理激活的标本中追踪表达的定位。未检测到组成性表达。在受刺激的标本中，这三个基因在Helix CNS中都有各自的表达模式，且表达水平依赖于刺激。表达感兴趣基因的细胞数量与投射到受刺激神经并因此逆行激活的细胞数量不同。这种差异取决于所研究的神经节。在亚细胞水平上，标记的RNA表现为点状（可能是RNA分子的小簇）和无形状的RNA团块，常在细胞核内边界处呈圆形。这些数据为进一步研究这些假定的即刻早期基因在蜗牛行为和学习中的行为作用提供了基础。
