Chen Ziqi, Liu Yang, Wang Qi, Fei Jianbo, Liu Xiangguo, Zhang Chuang, Yin Yuejia
Institute of Agricultural Biotechnology/Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China), Changchun 130033, China.
Curr Issues Mol Biol. 2024 Aug 15;46(8):8874-8889. doi: 10.3390/cimb46080524.
Soil salinization/alkalization is a complex environmental factor that includes not only neutral salt NaCl but also other components like NaCO. miRNAs, as small molecules that regulate gene expression post-transcriptionally, are involved in plant responses to abiotic stress. In this study, maize seedling roots were treated for 5 h with 100 mM NaCl, 50 mM NaCO, and HO, respectively. Sequencing analysis of differentially expressed miRNAs under these conditions revealed that the NaCO treatment group had the most differentially expressed miRNAs. Cluster analysis indicated their main involvement in the regulation of ion transport, binding, metabolism, and phenylpropanoid and flavonoid biosynthesis pathways. The unique differentially expressed miRNAs in the NaCl treatment group were related to the sulfur metabolism pathway. This indicates a significant difference in the response patterns of maize to different treatment groups. This study provides theoretical evidence and genetic resources for further analysis of the molecular mechanisms behind maize's salt-alkali tolerance.
土壤盐碱化是一个复杂的环境因素，不仅包括中性盐氯化钠，还包括其他成分如碳酸钠。微小RNA（miRNAs）作为在转录后调节基因表达的小分子，参与植物对非生物胁迫的响应。在本研究中，玉米幼苗根系分别用100 mM氯化钠、50 mM碳酸钠和水（此处原文HO有误，推测是H₂O）处理5小时。对这些条件下差异表达的miRNAs进行测序分析表明，碳酸钠处理组差异表达的miRNAs最多。聚类分析表明它们主要参与离子转运、结合、代谢以及苯丙烷类和黄酮类生物合成途径的调控。氯化钠处理组中独特的差异表达miRNAs与硫代谢途径有关。这表明玉米对不同处理组的响应模式存在显著差异。本研究为进一步分析玉米耐盐碱分子机制提供了理论依据和遗传资源。
