鉴定与胃癌预后相关的免疫细胞和 mRNA。
Identification of immune cells and mRNA associated with prognosis of gastric cancer.
机构信息
Department of Minimally Invasive Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, Hunan, China.
Department of Nursing, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
出版信息
BMC Cancer. 2020 Mar 12;20(1):206. doi: 10.1186/s12885-020-6702-1.
BACKGROUND
The clinical success demonstrates the enormous potential of immunotherapy in cancer treatment.
METHODS
This article presented research linking gastric cancer to immune cells, based on RNA-seq data of Stomach adenocarcinoma (STAD) and gene expression profile of GSE84437, 24 kinds of tumor-infiltrating immune cells were quantified by single-sample gene set enrichment analysis.
RESULTS
Th2 cells, T helper cells, and Mast cells were identified as prognostic immune cells in both TCGA and GEO groups. Then SUPV3L1 and SLC22A17 were identified as hub genes which may affect immune cell infiltration by correlation analysis. Survival analysis further proved that hub genes and prognostic immune cells are associated with the prognosis of gastric cancer. In gastrointestinal tumors, hub genes and prognostic immune cells also found differences in non-tumor and tumor tissues.
CONCLUSIONS
We found that three immune cells infiltration are associated with the prognosis of gastric cancer and further identify two hub genes. These two key genes may affect immune cell infiltration, result in the different prognosis of patients.
背景
临床成功证明了免疫疗法在癌症治疗中的巨大潜力。
方法
本文基于胃腺癌（STAD）的 RNA-seq 数据和 GSE84437 的基因表达谱，将胃癌与免疫细胞联系起来，通过单样本基因集富集分析对 24 种肿瘤浸润免疫细胞进行了量化。
结果
在 TCGA 和 GEO 两组中，均鉴定出 Th2 细胞、辅助性 T 细胞和肥大细胞是预后免疫细胞。然后通过相关性分析鉴定出 SUPV3L1 和 SLC22A17 为可能影响免疫细胞浸润的枢纽基因。生存分析进一步证明，枢纽基因和预后免疫细胞与胃癌的预后相关。在胃肠道肿瘤中，枢纽基因和预后免疫细胞在非肿瘤和肿瘤组织中也存在差异。
结论
我们发现三种免疫细胞浸润与胃癌的预后相关，并进一步鉴定出两个枢纽基因。这两个关键基因可能影响免疫细胞浸润，导致患者预后不同。
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