ABCA8 和 FABP4 在胃腺癌中的预后价值及免疫浸润分析。
Prognostic Value and Immune Infiltrates of ABCA8 and FABP4 in Stomach Adenocarcinoma.
机构信息
Department of Radiation Oncology, The Second Affiliated Hospital, Xi'an Jiao tong University, Xi'an, 710004 Shaanxi, China.
出版信息
Biomed Res Int. 2020 Jun 27;2020:4145164. doi: 10.1155/2020/4145164. eCollection 2020.
BACKGROUND
Stomach adenocarcinoma (STAD) is a common malignancy worldwide with poor prognosis. Therefore, it is important to identify a valuable prognostic biomarker for STAD. The aim of present study was to identify novel prognostic biomarkers for STAD and evaluate the potential role of hub genes in STAD.
METHODS
Gene Expression Profiling Interactive Analysis (GEPIA) and Cancer RNA-Seq Nexus were performed to identify differentially expressed genes (DEGs). Subsequently, hub genes were selected by a Venn diagram, and the expression of key genes was confirmed by UALCAN database. Furthermore, survival analysis of these hub genes was performed using Oncolnc and Human Protein Atlas (HPA) database. Gene alteration status of hub genes was assessed by cBioPortal. Finally, we investigated the association between hub genes and immune cell infiltration in STAD through the Tumor Immune Estimation Resource (TIMER) and GEPIA database.
RESULTS
Three common hub genes were obtained, including 2 downregulated DEGs (ABCA8 and FABP4) and one upregulated DEG (SLC52A3). Furthermore, increased expression of ABCA8 and FABP4 and decreased expression of SLC52A3 were correlated with poor prognosis. Meanwhile, three hub genes showed genetic alterations in various datasets of STAD. Finally, our results showed that ABCA8 and FABP4 displayed a positive correlation with immune infiltration, especially in M2 macrophages.
CONCLUSIONS
The results of this study suggest that ABCA8 and FABP4 may be used as prognostic biomarkers and correlated with immune infiltration in STAD.
背景
胃腺癌（STAD）是一种全球常见的恶性肿瘤，预后较差。因此，识别有价值的 STAD 预后生物标志物非常重要。本研究旨在识别 STAD 的新型预后生物标志物，并评估关键基因在 STAD 中的潜在作用。
方法
通过基因表达谱交互分析（GEPIA）和癌症 RNA-Seq 综合数据库（Cancer RNA-Seq Nexus）筛选差异表达基因（DEGs）。然后，通过 Venn 图选择枢纽基因，并通过UALCAN 数据库验证关键基因的表达。进一步通过 OncoLnc 和人类蛋白质图谱（Human Protein Atlas，HPA）数据库进行这些枢纽基因的生存分析。通过 cBioPortal 评估枢纽基因的基因改变状态。最后，通过肿瘤免疫估计资源（Tumor Immune Estimation Resource，TIMER）和 GEPIA 数据库研究枢纽基因与 STAD 中免疫细胞浸润的关系。
结果
获得了 3 个共同的枢纽基因，包括 2 个下调的 DEGs（ABCA8 和 FABP4）和 1 个上调的 DEG（SLC52A3）。此外，ABCA8 和 FABP4 表达增加以及 SLC52A3 表达减少与预后不良相关。同时，在不同的 STAD 数据集发现这 3 个枢纽基因存在遗传改变。最后，我们的结果表明，ABCA8 和 FABP4 与免疫浸润呈正相关，尤其是 M2 巨噬细胞。
结论
本研究结果表明，ABCA8 和 FABP4 可能作为 STAD 的预后生物标志物，与免疫浸润相关。
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