College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
College of Animal Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Maoming Agriculture&Forestry Technical College, Maoming 525000, China.
Gene. 2020 Jun 5;742:144550. doi: 10.1016/j.gene.2020.144550. Epub 2020 Mar 9.
Heat stress has a negative impact on dairy cow productivity. In order to reveal the mechanisms of heat-stress response, the mRNA and miRNA expression profiles of five cows under chronic heat-stress and thermoneutral conditions were assayed in blood by high-throughput sequencing technology. A total of 540 mRNAs and 9 miRNAs were expressed differently under heat-stress and thermoneutral conditions. Functional analyses revealed that MAPK signaling pathway, cellular senescence, circadian entrainment, aldosterone synthesis and secretion, and pathways in cancer were enriched for differently expressed mRNAs; meanwhile cGMP-PKG signaling pathway, thermogenesis, and protein digestion and absorption were enriched for differently expressed miRNAs. In addition, GADD45G, TGFB2, and GNG11 may play roles in the heat stress, and bta-miR-423-5p might be one of the regulators of heat-stress response in cows as potential mediators of chronic heat-stress response. In conclusion, the present study described the mRNA and miRNA expression patterns in blood extracted from cows during the transition from heat-stress to thermoneutral conditions. The results provide new data that could help in identifying mechanisms that mediate cows' response to chronic heat stress.
热应激对奶牛生产性能有负面影响。为了揭示热应激反应的机制，本研究采用高通量测序技术检测了 5 头奶牛在慢性热应激和常温条件下血液中的 mRNA 和 miRNA 表达谱。在热应激和常温条件下，共有 540 个 mRNAs 和 9 个 miRNAs 表达不同。功能分析显示，MAPK 信号通路、细胞衰老、昼夜节律同步、醛固酮合成和分泌以及癌症相关通路中差异表达的 mRNAs 富集；而 cGMP-PKG 信号通路、产热和蛋白质消化吸收等通路中差异表达的 miRNAs 富集。此外，GADD45G、TGFB2 和 GNG11 可能在热应激中发挥作用，bta-miR-423-5p 可能作为慢性热应激反应的潜在调节剂之一，参与奶牛的热应激反应。总之，本研究描述了奶牛从热应激到常温条件过渡过程中血液中提取的 mRNA 和 miRNA 表达模式。研究结果提供了新的数据，有助于确定介导奶牛对慢性热应激反应的机制。
