Department of Environmental Health Science, College of Public Health, University of Georgia, Athens, GA, USA.
Gene. 2020 May 25;740:144540. doi: 10.1016/j.gene.2020.144540. Epub 2020 Mar 9.
Harpalus pensylvanicus (Coloptera: Carabidae) is a weed seed predator common throughout the United States. While Carabidae is a very large group of beetles, limited genomic resources exist, especially mitochondrial genomes. This study expands research in this area by assembling and annotating the complete mitochondrial genome of H. pensylvanicus and performs phylogenetic analyses with closely related species. Further use of the metagenomic data was made to characterize microbial taxa and clusters of orthologous groups of proteins. The complete mitochondrial genome is 16,434 bp in length, AT rich, and consist of 13 protein coding genes, 2 ribosomal RNAs, 22 transfer RNAs, and a control region. Phylogenetic analyses were congruent with the Harpalinae and Pterostichinae clade together. Microbial classification shows a predominance of Gamma- (37.77%) and Alpha-proteobacteria (33.97%).
宾夕法尼亚弓背蚁（鞘翅目：步甲科）是一种普遍存在于美国的杂草种子捕食者。虽然步甲科是一个非常大的甲虫群，但现有的基因组资源有限，特别是线粒体基因组。本研究通过组装和注释宾夕法尼亚弓背蚁的完整线粒体基因组，并与亲缘关系密切的物种进行系统发育分析，扩展了该领域的研究。进一步利用宏基因组数据来描述微生物分类群和直系同源基因簇。完整的线粒体基因组长 16434bp，富含 AT，由 13 个蛋白质编码基因、2 个核糖体 RNA、22 个转移 RNA 和一个控制区组成。系统发育分析与弓背蚁亚科和步甲亚科聚类一致。微生物分类显示出γ-（37.77%）和α-变形菌（33.97%）的优势。
