College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China.
Technical Center, Zhengzhou Customs District, Zhengzhou 450002, China.
Genes (Basel). 2020 Feb 9;11(2):181. doi: 10.3390/genes11020181.
Carabidae are one of the most species-rich families of beetles, comprising more than 40,000 described species worldwide. Forty-three complete or partial mitochondrial genomes (mitogenomes) from this family have been published in GenBank to date. In this study, we sequenced a nearly complete mitogenome of (Carabidae), using a next-generation sequencing method. This mitogenome was 16,646 bp in length, which encoded the typical 13 mitochondrial protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and a putative control region. Combining with the published mitogenomes of Carabidae and five outgroup species from Trachypachidae, Gyrinidae and Dytiscidae, we performed phylogenetic estimates under maximum likelihood and Bayesian inference criteria to investigate the phylogenetic relationships of carabid beetles. The results showed that the family Carabidae was a non-monophyletic assemblage. The subfamilies Cicindelinae, Elaphrinae, Carabinae, Trechinae and Harpalinae were recovered as monophyletic groups. Moreover, the clade (Trechinae + (Brachininae + Harpalinae)) was consistently recovered in all analyses.
步甲科是甲虫中物种最丰富的科之一，全世界已描述的物种超过 40,000 种。迄今为止，GenBank 已发表了该科的 43 个完整或部分线粒体基因组（mitogenome）。在这项研究中，我们使用下一代测序方法对 （Carabidae）的近乎完整的线粒体基因组进行了测序。该线粒体基因组长 16646bp，编码了典型的 13 个线粒体蛋白编码基因、22 个转移 RNA 基因、两个核糖体 RNA 基因和一个假定的控制区。结合已发表的步甲科和来自 Trachypachidae、Gyrinidae 和 Dytiscidae 的五个外群物种的 mitogenomes，我们在最大似然和贝叶斯推断标准下进行了系统发育估计，以研究步甲科甲虫的系统发育关系。结果表明，步甲科不是一个单系群。拟步甲亚科、萤甲亚科、步甲亚科、锯角叶甲亚科和虎甲亚科被恢复为单系群。此外，在所有分析中，（锯角叶甲亚科+（扁甲亚科+虎甲亚科））支系都得到了一致的恢复。
