Stem Cell and Molecular Biology Laboratory, Bhupat and Jyoti Mehta School of Biosciences, Department of Biotechnology, Indian Institute of Technology Madras, Chennai TN-600036, India.
Stem Cell and Molecular Biology Laboratory, Bhupat and Jyoti Mehta School of Biosciences, Department of Biotechnology, Indian Institute of Technology Madras, Chennai TN-600036, India; Heat Transfer and Thermal Power Laboratory, Department of Mechanical Engineering, Indian Institute of Technology Madras, Chennai TN-600036, India.
Drug Discov Today. 2020 May;25(5):879-890. doi: 10.1016/j.drudis.2020.03.002. Epub 2020 Mar 9.
Successful translation of potential cancer chemotherapeutic drugs to the clinic depends on sufficient predictability of response in the human system through in vitro simulations. High expenditure and longer duration in preclinical and clinical research urge the enhancement of effective in vitro drug screening. 3D models emulate cell morphology, cell-cell and cell-matrix interactions and are physiologically more relevant for predicting drug responses for complex heterogenic cancers, widely replacing conventional cultures. Bioprinting and microfluidic technology facilitate tissue mimetic model construction and multifaceted simulation of physiology, respectively, promising more-appropriate predictability of drug interactions. Precisely, organotypic tissue constructs assembled using cell-laden matrices or organ-on-a-chip serve as realistic tissue models. This review projects the progress toward biomimetic tissue model development, highlighting the emergence of bioprinting and microfluidic technology in in vitro cancer drug screening and pertaining challenges.
成功将潜在的癌症化疗药物转化为临床应用取决于通过体外模拟在人体系统中对反应有足够的可预测性。临床前和临床研究的高支出和更长的持续时间促使人们需要增强有效的体外药物筛选。3D 模型模拟细胞形态、细胞-细胞和细胞-基质相互作用，并且在预测复杂异质癌症的药物反应方面更具生理相关性，广泛替代传统培养物。生物打印和微流控技术分别促进组织模拟模型的构建和多方面的生理学模拟，有望提高药物相互作用的预测准确性。具体来说，使用细胞负载基质或器官芯片组装的器官型组织构建体可作为现实的组织模型。这篇综述展示了仿生组织模型发展的进展，重点介绍了生物打印和微流控技术在体外癌症药物筛选中的出现及其相关挑战。
