Qu Baozhen, Mu Qiang, Bi Huanhuan, Chen Yuxian, Wang Qitang, Ma Xuezhen, Lu Linlin
Qingdao Cancer Prevention and Treatment Research Institute, Qingdao Central Hospital, University of Health and Rehabilitation Sciences (Qingdao Central Hospital), Qingdao, China.
The First Department of Breast Surgery, Qingdao Central Hospital, University of Health and Rehabilitation Sciences (Qingdao Central Hospital), Qingdao, China.
Front Cell Dev Biol. 2024 Aug 13;12:1433111. doi: 10.3389/fcell.2024.1433111. eCollection 2024.
Organoid technology has been developed rapidly in the past decade, which involves the exploration of the mechanism of development, regeneration and various diseases, and intersects among multiple disciplines. Thousands of literature on 3D-culture or organoids have been published in the research areas of cell biology tissue engineering, nanoscience, oncology and so on, resulting in it being challenging for researchers to timely summarize these studies. Bibliometric statistics is a helpful way to help researchers clarify the above issues efficiently and manage the whole landscape systematically. In our study, all original articles on organoids were included in the Web of Science database from January 2009 to May 2024, and related information was collected and analyzed using Excel software, "bibliometrix" packages of the R software, VOSviewer and CiteSpace. As results, a total of 6222 papers were included to classify the status quo of the organoids and predict future research areas. Our findings highlight a growing trend in publications related to organoids, with the United States and Netherlands leading in this field. The University of California System, Harvard University, Utrecht University and Utrecht University Medical Center have emerged as pivotal contributors and the key authors in the field include Clevers, H, Beekman, JM and Spence JR. Our results also revealed that the research hotspots and trends of organoids mainly focused on clinical treatment, drug screening, and the application of materials and technologies such as "hydrogel" and "microfluidic technology" in organoids. Next, we had an in-depth interpretation of the development process of organoid research area, including the emergence of technology, the translation from bench to bedsides, the profiles of the most widely studied types of organoids, the application of materials and technologies, and the emerging organoid-immune co-cultures trends. Furthermore, we also discussed the pitfalls, challenges and prospects of organoid technology. In conclusion, this study provides readers straightforward and convenient access to the organoid research field.
在过去十年中，类器官技术发展迅速，它涉及对发育、再生及各种疾病机制的探索，且跨越多个学科。在细胞生物学、组织工程、纳米科学、肿瘤学等研究领域，已发表了数千篇关于三维培养或类器官的文献，这使得研究人员难以及时总结这些研究。文献计量学统计是帮助研究人员有效阐明上述问题并系统管理整体情况的有用方法。在我们的研究中，将2009年1月至2024年5月期间Web of Science数据库中所有关于类器官的原创文章纳入研究，使用Excel软件、R软件的“bibliometrix”包、VOSviewer和CiteSpace收集并分析相关信息。结果，共纳入6222篇论文以分类类器官的现状并预测未来研究领域。我们的研究结果突出了与类器官相关出版物的增长趋势，美国和荷兰在该领域领先。加利福尼亚大学系统、哈佛大学、乌得勒支大学和乌得勒支大学医学中心已成为关键贡献者，该领域的关键作者包括克莱弗斯、H、比克曼、JM和斯彭斯JR。我们的结果还表明，类器官的研究热点和趋势主要集中在临床治疗、药物筛选以及“水凝胶”和“微流控技术”等材料和技术在类器官中的应用。接下来，我们深入解读了类器官研究领域的发展过程，包括技术的出现、从实验室到临床的转化、研究最广泛的类器官类型概况、材料和技术的应用以及新兴的类器官 - 免疫共培养趋势。此外，我们还讨论了类器官技术的陷阱、挑战和前景。总之，本研究为读者提供了直接且便捷地了解类器官研究领域的途径。
