Ozturk Ismail, Tunçel Ayça, Yurt Fatma, Biyiklioglu Zekeriya, Ince Mine, Ocakoglu Kasim
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Izmir Katip Celebi University, Izmir, 35620, Turkey.
Institute of Nuclear Science, Department of Nuclear Applications, Ege University, Izmir, 35100, Turkey.
Photodiagnosis Photodyn Ther. 2020 Jun;30:101715. doi: 10.1016/j.pdpdt.2020.101715. Epub 2020 Mar 9.
Antimicrobial resistance is one of the most important causes of morbidity and mortality in the treatment of infectious diseases worldwide. Candida albicans is one of the most virulent and common species of fungi to cause invasive fungal infections on humans. Alternative treatment strategies, including photodynamic therapy, are needed for controlling these infectious diseases. The aim of this study was to investigate the antifungal photodynamic activities of phthalocyanine derivatives on C. albicans. The minimum inhibitory concentration (MIC) values of compounds were determined by the broth microdilution method. Uptake of the compounds in C. albicans and dark toxicity of the compounds were also investigated. Photodynamic inhibition of growth experiments was performed by measuring the colony-forming unit/mL (CFU/mL) of the strain. Maximum uptake into the cells was observed in the presence of 64 μg/mL concentration for each compound except for ZnPc. Compounds did not show dark toxicity/inhibitory effects at sub-MIC concentrations on C. albicans when compared to the negative control groups. Zn(II)Pc, ZnPc, and ZnPc-TiO showed fungicidal effect after irradiation with the light dose of 90 J/cm in the presence of the compounds. In addition to the fungicidal effects, SubPc, SubPc-TiO, Es-SiPc, and Es-SubPc compounds were also found to have inhibitory effects on the growth of yeast cells after irradiation.
抗菌药物耐药性是全球传染病治疗中发病和死亡的最重要原因之一。白色念珠菌是导致人类侵袭性真菌感染的最具毒性和最常见的真菌物种之一。需要包括光动力疗法在内的替代治疗策略来控制这些传染病。本研究的目的是研究酞菁衍生物对白色念珠菌的抗真菌光动力活性。通过肉汤微量稀释法测定化合物的最低抑菌浓度（MIC）值。还研究了化合物在白色念珠菌中的摄取情况以及化合物的暗毒性。通过测量菌株的菌落形成单位/毫升（CFU/mL）进行光动力生长抑制实验。除了锌酞菁（ZnPc）外，每种化合物在浓度为64μg/mL时观察到最大细胞摄取量。与阴性对照组相比，化合物在低于MIC浓度时对白色念珠菌未显示暗毒性/抑制作用。在化合物存在的情况下，用90 J/cm的光剂量照射后，锌（II）酞菁（Zn(II)Pc）、锌酞菁（ZnPc）和锌酞菁 - 二氧化钛（ZnPc-TiO）显示出杀菌作用。除了杀菌作用外，亚酞菁（SubPc）、亚酞菁 - 二氧化钛（SubPc-TiO）、乙氧基硅酞菁（Es-SiPc）和乙氧基亚酞菁（Es-SubPc）化合物在照射后也被发现对酵母细胞的生长具有抑制作用。
