DiCesare Nicolas, Pinto Mauricio R, Schanze Kirk S, Lakowicz Joseph R
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, Maryland 21201.
Department of Chemistry, University of Florida, Gainesville, Florida 32611.
Langmuir. 2002 Oct 1;18(21):7785-7787. doi: 10.1021/la0264926. Epub 2002 Sep 13.
The amplified quenching of an anionic conjugated polymer, sulfonated poly(phenylene ethynylene) (PPE-SO ), by a cationic quencher comprising a boronic acid functionalized benzyl viologen (-BV), has been used to optically detect sugars. In the absence of sugar, a strong polymer/quencher interaction leads to superlinear quenching. In the presence of sugar at pH 7.4, the dicationic viologen derivative forms a neutral zwitterionic species, reducing its ability to complex with and quench the anionic polymer's emission. Addition of sugar to the polymer/quencher system leads a large increase in the fluorescence intensity (up to 70-fold in one case). Spectral data, quenching parameters, and sugar titration curves are presented and discussed in terms of future development of glucose sensors.
一种包含硼酸功能化苄基紫精（-BV）的阳离子猝灭剂对阴离子共轭聚合物磺化聚（亚苯基亚乙炔）（PPE-SO）的增强猝灭作用已被用于光学检测糖类。在无糖情况下，聚合物/猝灭剂之间的强相互作用导致超线性猝灭。在pH 7.4且存在糖的情况下，二价阳离子紫精衍生物形成中性两性离子物种，降低了其与阴离子聚合物络合并猝灭其发射的能力。向聚合物/猝灭剂体系中添加糖会导致荧光强度大幅增加（在一种情况下高达70倍）。根据葡萄糖传感器的未来发展，给出并讨论了光谱数据、猝灭参数和糖滴定曲线。
