Institute of Ensiling and Processing of Grass, College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing, China.
J Appl Microbiol. 2020 Sep;129(3):509-520. doi: 10.1111/jam.14636. Epub 2020 Apr 12.
To evaluate the effects of various epiphytic microbiota from Italian ryegrass (IR), maize (MZ) and sorghum (SG) on fermentative profile and microbial community dynamics in sterile IR.
Using microbiota transplantation, the irradiated IR was treated with the following: (i) sterile water; (ii) epiphytic microbiota on IR (IRIR); (iii) epiphytic microbiota on MZ (IRMZ); (iv) epiphytic microbiota on SG (IRSG). After 60 days of ensiling, MZ and SG microbiota significantly (P < 0·05) decreased lactic acid (LA) and acetic acid (AA) concentrations compared to IR microbiota, while SG microbiota notably (P < 0·05) reduced the ratio of LA to AA than MZ and IR microbiota. Apparently (P < 0·01) higher amounts of Lactobacillus genus were observed in IRIR and IRMZ groups on 60 day compared to IRSG group, and the dominant Lactococcus genus on 3 day was eventually replaced by Enterobacteriaceae and Lactobacillus in IRSG group.
Exogenous microbiota could evidently affect the fermentative profile and microbial community dynamics of IR silage. The numbers of Enterobacteriaceae and Lactobacillus were mainly responsible for this.
Identifying the role of microbe during ensiling is of great significance to manipulate the fermentation products and improve the preservation of silage.
评估意大利黑麦草（IR）、玉米（MZ）和高粱（SG）的各种附生微生物群落对无菌 IR 发酵特性和微生物群落动态的影响。
使用微生物群落移植，用以下方法处理辐照 IR：（i）无菌水；（ii）IR 上的附生微生物群落（IRIR）；（iii）MZ 上的附生微生物群落（IRMZ）；（iv）SG 上的附生微生物群落（IRSG）。青贮 60 天后，与 IR 微生物群落相比，MZ 和 SG 微生物群落显著（P<0.05）降低了乳酸（LA）和乙酸（AA）浓度，而 SG 微生物群落显著（P<0.05）降低了 LA 与 AA 的比例比 MZ 和 IR 微生物群落。显然（P<0.01），与 IRSG 组相比，IRIR 和 IRMZ 组在 60 天时乳杆菌属的数量明显更高，而在 3 天时占主导地位的乳球菌属最终被肠杆菌科和乳杆菌属取代。
外源性微生物群落可以明显影响 IR 青贮的发酵特性和微生物群落动态。肠杆菌科和乳杆菌属的数量主要负责这一点。
在青贮过程中识别微生物的作用对于操纵发酵产物和提高青贮的保存具有重要意义。
