Institute of Ensiling and Processing of Grass, College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing 210095, China.
Institute of Ensiling and Processing of Grass, College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing 210095, China.
Bioresour Technol. 2020 Aug;309:123371. doi: 10.1016/j.biortech.2020.123371. Epub 2020 Apr 11.
This study was aimed to assess the effects of exogenous microbiota on fermentation quality and bacterial community dynamics in oat (OT) silage. The irradiated OT was treated with the following: (i) sterile water (STOT); (ii) epiphytic microbiota on oat (OTOT); (iii) epiphytic microbiota on maize (OTMZ); (iv) epiphytic microbiota on sorghum (OTSG). γ-Ray irradiation, microbiota transplantation and sequencing methods were firstly used. After 60 days of ensiling, OTMZ group had higher lactic acid (LA) and lower acetic acid (AA) contents than OTOT group. Inversely, lower LA content and higher ratio of LA to AA was observed in OTSG group than OTOT group. Lactobacillus was predominant in OTMZ-60 group, while Lactobacillus and Enterobacteriaceae were both dominant in OTSG-60 group. Overall, the higher amounts of Enterobacteriaceae and heterofermentative Lactobacillus are conductive to acetic acid-type fermentation, and forage microbiota transplantation may be a potential way to identify the role of microbe during ensiling.
本研究旨在评估外源性微生物群落对燕麦青贮发酵品质和细菌群落动态的影响。辐照后的燕麦分别用以下方法处理：（i）无菌水（STOT）；（ii）燕麦附生微生物（OTOT）；（iii）玉米附生微生物（OTMZ）；（iv）高粱附生微生物（OTSG）。首先采用γ射线辐照、微生物移植和测序方法。青贮 60 天后，OTMZ 组的乳酸（LA）含量较高，乙酸（AA）含量较低，而 OTSG 组的 LA 含量较低，LA 与 AA 的比值较高。在 OTMZ-60 组中，乳杆菌占优势，而在 OTSG-60 组中，乳杆菌和肠杆菌科均占优势。总的来说，肠杆菌科和异型发酵乳杆菌的含量较高有利于乙酸型发酵，而饲料微生物移植可能是鉴定青贮过程中微生物作用的一种潜在方法。
