Nazar Mudasir, Ullah Muhammad Wajid, Wang Siran, Zhao Jie, Dong Zhihao, Li Junfeng, Kaka Niaz Ali, Shao Tao
Institute of Ensiling and Processing of Grass, College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing 210095, China.
Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
Bioengineering (Basel). 2022 Aug 30;9(9):428. doi: 10.3390/bioengineering9090428.
In this study, the effects of epiphytic microbiota from different forages on the fermentation characteristics and microbial community structure of forage sorghum silage were investigated. The gamma irradiated sterilized forage sorghum was treated through sterile water, epiphytic microbiota of forage sorghum (FSm), Sudan grass (SDm), Napier grass (NPm), and maize (MZm). NPm and SDm inoculated silages showed similar pH value and lactic acid (LA) and acetic acid (AA) contents at day 3 and 60 of ensiling. The final silage of FSm and MZm showed lower (p < 0.05) pH and AA content and a higher LA content compared to the NPm and SDm silages. Bacterial species from the Weisella genus were predominantly present in FSm, NPm, and SDm, while Lactococcus dominated the MZm silage during early ensiling. Lactobacillus was predominant in all inoculated terminal silages. Overall, the four inoculated microbiota decreased the pH value of silage and were dominated by lactic acid bacteria (LAB); however, the NPm and SDm treatments resulted in comparatively higher AA contents which could have an inhibitory effect on the secondary fermentation developed by the yeast and enhanced the aerobic stability of forage sorghum silage.
本研究考察了不同草料附生微生物群对饲用高粱青贮发酵特性和微生物群落结构的影响。用γ射线辐照灭菌的饲用高粱分别用无菌水、饲用高粱（FSm）、苏丹草（SDm）、象草（NPm）和玉米（MZm）的附生微生物群进行处理。接种NPm和SDm的青贮饲料在青贮第3天和第60天时，pH值、乳酸（LA）和乙酸（AA）含量相似。与接种NPm和SDm的青贮饲料相比，接种FSm和MZm的最终青贮饲料pH值和AA含量较低（p<0.05），LA含量较高。魏斯氏菌属细菌主要存在于FSm、NPm和SDm中，而在青贮前期，乳球菌在接种MZm的青贮饲料中占主导地位。在所有接种的最终青贮饲料中，乳酸菌占主导。总体而言，四种接种的微生物群均降低了青贮饲料的pH值，且以乳酸菌（LAB）为主；然而，NPm和SDm处理导致AA含量相对较高，这可能会抑制酵母引发的二次发酵，并提高饲用高粱青贮饲料的有氧稳定性。
