Fujian Provincial Key Laboratory of Innovative Drug Target, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, People's Republic of China.
Fujian Provincial Key Laboratory of Neurodegenerative Disease and Aging Research, Institute of Neuroscience, School of Medicine, Xiamen University, Xiamen 361102, People's Republic of China.
J Agric Food Chem. 2020 Apr 8;68(14):4196-4204. doi: 10.1021/acs.jafc.9b07876. Epub 2020 Mar 26.
Vahl. (Wuzhimaotao) is an edible functional food used for the soup cooking and health products. Seven undescribed phenolic glycosides (-), along with 20 analogues, were isolated from the roots of . Their structures were determined by comprehensive spectroscopic methods (UV, IR, HRESIMS, and NMR), while the absolute configuration of was established by comparison of the experimental and calculated ECD data. The antineuroinflammatory effects of all the compounds were examined by Western blot. Compounds and attenuated the phosphorylation of AKT, JNK, and ERK1/2. In addition, compound inhibited the NF-κB p65 phosphorylation. Our results indicated that compounds and decreased the occurrence of neuroinflammation in BV2 microglia cells, which might be regulated by inhibiting the activity of proteins in NF-κB, MAPK (JNK and ERK1/2), or AKT signaling pathways. Thus, and might exhibit antineuroinflammatory activities and show promise in treating neurodegenerative diseases.
瓦赫氏鼠李（菝葜）是一种可食用的功能性食品，可用于煲汤和制作保健品。从菝葜的根部分离得到了 7 种未描述的酚糖苷（-），以及 20 种类似物。通过综合光谱方法（UV、IR、HRESIMS 和 NMR）确定了它们的结构，而 通过比较实验和计算的 ECD 数据确定了 的绝对构型。通过 Western blot 检测了所有化合物的抗神经炎症作用。化合物 和 可减弱 AKT、JNK 和 ERK1/2 的磷酸化。此外，化合物 抑制 NF-κB p65 的磷酸化。研究结果表明，化合物 和 可降低 BV2 小胶质细胞中神经炎症的发生，这可能是通过抑制 NF-κB、MAPK（JNK 和 ERK1/2）或 AKT 信号通路中的蛋白质活性来调节的。因此， 和 可能具有抗神经炎症活性，有望用于治疗神经退行性疾病。
