He Qianqian, Lu Anjing, Qin Lin, Zhang Qianru, Lu Yanliu, Yang Zhou, Tan Daopeng, He Yuqi
Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, School of Pharmacy, Zunyi Medical University, Zunyi 563000, Guizhou, China.
Shanghai Nature-Standard Technical Service Co.,Ltd, Shanghai 201203, China.
J Anal Methods Chem. 2022 Nov 4;2022:8026410. doi: 10.1155/2022/8026410. eCollection 2022.
() is a valuable traditional Chinese herbal medicine with high commercial value. In (Ch.P., 2020 edition), the quality of is mainly evaluated by its polysaccharide content. However, varying growth and production conditions, such as cultivation environment, origin, harvesting process, or processing methods, resulting in highly variable yields, quality, and composition. The aim of this study was to investigate whether the content of secondary metabolites in from different origins is consistent with the polysaccharide content. The results showed that the polysaccharide content and pass rate were ranked as GX > AH > GZ > YN. Based on the nontargeted metabolomics approach, we searched for differential components in 22 different regions of , including amides, bibenzyls, disaccharide, flavonoids, organic nitrogenous compounds, and phenolic glycosides. The overall expression was opposite to the polysaccharide, and the most expressed was YN, followed by GZ, AH, and GX. These results indicated that the current quality standard for evaluating the quality of by polysaccharide content alone is imperfect, and small molecule compounds need to be included as quality markers.
（）是一种具有高商业价值的珍贵传统中草药。在《中国药典》2020年版中，（）的质量主要通过其多糖含量来评估。然而，不同的生长和生产条件，如栽培环境、产地、采收过程或加工方法，导致产量、质量和成分差异很大。本研究的目的是调查不同产地（）中次生代谢产物的含量是否与多糖含量一致。结果表明，多糖含量和合格率排序为GX＞AH＞GZ＞YN。基于非靶向代谢组学方法，我们在（）的22个不同产地中寻找差异成分，包括酰胺类、联苄类、二糖、黄酮类、有机含氮化合物和酚苷类。总体表达与多糖相反，表达最多的是YN，其次是GZ、AH和GX。这些结果表明，目前仅通过多糖含量来评价（）质量的质量标准不完善，需要将小分子化合物纳入质量标志物。
