Medicity Research Laboratory, Faculty of Medicine, University of Turku, Turku, Finland.
Department of Biochemistry and Biotechnology, University Rovira i Virgili, Tarragona, Catalonia, Spain.
Sci Prog. 2024 Apr-Jun;107(2):368504241244666. doi: 10.1177/00368504241244666.
Bone extracellular matrix (ECM) proteins play a key role in bone formation and regeneration, including structural and regulatory functions. The Phylobone database consists of 255 ECM protein groups from 39 species and can be used to support bone research. Here, we gathered bone ECM proteins from reindeer (), a member of the Cervidae family. The importance of reindeer lies in their ability to regenerate their antlers, in both male and female individuals. Protein sequences were extracted from the National Center for Biotechnology Information's repository and selected by homology searches. We identified 215 proteins and their corresponding functional domains, which are putatively present in the bone ECM of reindeer. Protein sequence alignments have shown a high degree of conservation between and other members of the Cervidae family. This update expands the Phylobone database and shows that it is a useful resource for the preliminary annotation of bone ECM proteins in novel proteomes.
骨细胞外基质 (ECM) 蛋白在骨形成和再生中发挥着关键作用，包括结构和调节功能。Phylobone 数据库包含来自 39 个物种的 255 个 ECM 蛋白组，可用于支持骨研究。在这里，我们从鹿科（Cervidae）的一种动物驯鹿（）中收集了骨 ECM 蛋白。驯鹿的重要性在于它们能够再生鹿角，无论是雄性还是雌性个体。从国家生物技术信息中心（National Center for Biotechnology Information）的存储库中提取蛋白质序列，并通过同源搜索进行选择。我们鉴定了 215 种蛋白质及其对应的功能结构域，这些结构域可能存在于驯鹿的骨 ECM 中。蛋白质序列比对表明，与鹿科的其他成员之间存在高度的保守性。本次更新扩展了 Phylobone 数据库，并表明它是对新蛋白质组中骨 ECM 蛋白进行初步注释的有用资源。
