Kozlowska Lucyna, Mizera Olga, Mroz Anna
Department of Dietetics, Faculty of Human Nutrition, Warsaw University of Life Sciences - WULS, 02-776 Warsaw, Poland.
Department of Physiology and Sport Medicine, Jozef Pilsudski University of Physical Education in Warsaw, 00-968 Warsaw, Poland.
Metabolites. 2020 Mar 11;10(3):100. doi: 10.3390/metabo10030100.
This study aimed at assessment of the long-term (4 weeks) metabolic effect of a diet with and without beetroot juice supplementation in fencers using the untargeted metabolomics method with the UPLC Q-TOF/MS system to carry out an analysis of urine samples. Ten women and 10 men underwent the cardiovascular fitness VO test at baseline-(B) and after two stages of implementation of the dietary recommendations-the first 4 weeks without beetroot juice (D) and the second with 26 g/d of freeze-dried beetroot juice supplementation (D&J). The urine samples were collected one hour after the VO test at B and after D and D&J. The meal before the VO test after D&J contained beetroot juice, whereas to the meal at B and after D maltodextrin was added. Changes in metabolites and VO were significant only for comparison of D versus D&J. During D and D&J, there were no significant changes in the physical activity level, body mass, and body composition. We observed significant changes in tyrosine and tryptophan metabolism, mainly associated with such neurotransmitter's metabolism as: Serotonin, noradrenaline, and adrenaline. Changes in signal intensity of bile acid, AICAR, and 4-Hydroxynonenal (peroxidation of polyunsaturated fatty acids product) were also observed. The obtained results indicate that long-term beetroot juice supplementation induces considerable changes in metabolism.
本研究旨在通过超高效液相色谱-四极杆飞行时间质谱（UPLC Q-TOF/MS）系统采用非靶向代谢组学方法，分析击剑运动员饮用和不饮用甜菜根汁的饮食的长期（4周）代谢效应，对尿液样本进行分析。10名女性和10名男性在基线（B）时以及在实施饮食建议的两个阶段后，即第一阶段4周不饮用甜菜根汁（D），第二阶段每天补充26克冻干甜菜根汁（D&J）后，进行了心血管适能VO测试。在B、D和D&J阶段的VO测试后1小时收集尿液样本。D&J阶段VO测试前的餐食中含有甜菜根汁，而在B阶段和D阶段后的餐食中添加了麦芽糊精。仅在比较D和D&J时，代谢物和VO的变化具有显著性。在D和D&J阶段，身体活动水平、体重和身体成分没有显著变化。我们观察到酪氨酸和色氨酸代谢有显著变化，主要与血清素、去甲肾上腺素和肾上腺素等神经递质的代谢有关。还观察到胆汁酸、AICAR和4-羟基壬烯醛（多不饱和脂肪酸过氧化产物）信号强度的变化。所得结果表明，长期补充甜菜根汁会引起代谢的显著变化。
