Private Practice, Via Nizza, 46, 00198 Rome, Italy.
Department of Life Sciences and Systems Biology, University of Torino, 10126 Turin, Italy.
Int J Mol Sci. 2020 Mar 11;21(6):1919. doi: 10.3390/ijms21061919.
Attaining an effective mucosal attachment to the transmucosal part of the implant could protect the peri-implant bone.
To evaluate if chair side surface treatments (plasma of Argon and ultraviolet light) may affect fibroblast adhesion on different titanium surfaces designed for soft tissue healing.
Grade 5 titanium discs with four different surface topographies were subdivided into 3 groups: argon-plasma; ultraviolet light, and no treatment. Cell morphology and adhesion tests were performed at 20 min, 24 h, and 72 h.
Qualitative observation of the surfaces performed at the SEM was in accordance with the anticipated features. Roughness values ranged from smooth (MAC Sa = 0.2) to very rough (XA Sa = 21). At 20 min, all the untreated surfaces presented hemispherical cells with reduced filopodia, while the cells on treated samples were more spread with broad lamellipodia. However, these differences in spreading behavior disappeared at 24 h and 72 h. Argon-plasma, but not UV, significantly increased the number of fibroblasts independently of the surface type but only at 20 min. Statistically, there was no surface in combination with a treatment that favored a greater cellular adhesion.
Data showed potential biological benefits of treating implant abutment surfaces with the plasma of argon in relation to early-stage cell adhesion.
实现种植体穿黏膜部分与黏膜的有效附着可以保护种植体周围骨。
评估椅旁表面处理（氩气等离子体和紫外线）是否会影响不同钛表面上纤维细胞的黏附，这些钛表面是为软组织愈合设计的。
将具有四种不同表面形貌的 5 级钛片分为 3 组：氩等离子体组、紫外线组和未处理组。在 20 分钟、24 小时和 72 小时进行细胞形态和黏附试验。
SEM 表面定性观察符合预期特征。粗糙度值从光滑（MAC Sa = 0.2）到非常粗糙（XA Sa = 21）不等。在 20 分钟时，所有未经处理的表面均呈现出具有减少丝状伪足的半球形细胞，而处理样品上的细胞则具有更宽的片状伪足，更舒展。然而，在 24 小时和 72 小时，这些伸展行为的差异消失了。氩等离子体，但不是紫外线，独立于表面类型，仅在 20 分钟时显著增加了成纤维细胞的数量。从统计学上讲，没有一种表面与处理方式相结合可以促进更大的细胞黏附。
数据显示，用氩气等离子体处理种植体基台表面在早期细胞黏附方面具有潜在的生物学益处。
