CIR Dental School, Department of Surgical Sciences, University of Torino, via Nizza 230, 10126, Turin, Italy.
Department of Life Sciences and Systems Biology, University of Torino, via Accademia Albertina 13, 10123, Turin, Italy.
J Biomed Mater Res A. 2019 Jan;107(1):67-70. doi: 10.1002/jbm.a.36531. Epub 2018 Oct 25.
Plasma of argon was demonstrated to improve protein and cell adhesion on bone grafts. On the other hand, increased surface energy and hydrophilicity could potentially amplify the risks of graft surface contamination in a clinical environment. The aim of the present study was to in vitro verify if the plasma of argon treatment could alter the graft characteristics affecting its ability to remain sterile. Six graft materials produced by different company were selected for this study, and randomly split by allocation either in the test (Plasma of argon treatment for 20') or the control group (only removed from the plastic sterile vials). To replicate the surgical work flow, both test and control samples were left 2 min in the clinical environment simulated conditions. Samples were therefore transferred in a Biosafety level 2 culture room. Bacterial growth analysis was performed. Optical density at 600 nm was measured as readout of bacterial growth and, after 24 hours, colony forming unit (CFU) was evaluated. Statistical analysis was performed by using the ordinary one-way ANOVA. The optical density confirmed no significant differences within groups and the number of CFU/ml for each measured sample (test and control) failed to present significant differences. Data from the present study highlighted that surface activation using plasma of argon did not affect the degree of contamination of the bone grafts, allowing to maintain a required sterility of the surface. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 67-70, 2019.
氩等离子体被证明可改善移植物上的蛋白质和细胞黏附。另一方面，表面能和亲水性的增加可能会增加移植物表面在临床环境中受到污染的风险。本研究的目的是体外验证氩等离子体处理是否会改变移植物的特性，从而影响其保持无菌的能力。选择了由不同公司生产的 6 种移植物材料进行本研究，并通过分配随机分为实验组（氩等离子体处理 20 分钟）或对照组（仅从无菌塑料小瓶中取出）。为了复制手术工作流程，实验组和对照组样本均在模拟临床环境条件下放置 2 分钟。然后将样本转移到生物安全 2 级培养室中。进行细菌生长分析。测量 600nm 处的吸光度作为细菌生长的读数，24 小时后，评估集落形成单位（CFU）。通过使用普通单因素方差分析进行统计分析。吸光度证实组内无显著差异，每个测量样本（实验组和对照组）的 CFU/ml 数量也无显著差异。本研究的数据表明，使用氩等离子体进行表面活化不会影响骨移植物的污染程度，从而保持表面所需的无菌性。2018 年 Wiley 期刊公司。J 生物医学材料研究杂志 A 部分：107A：67-70，2019 年。
