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A method for parallel determination of choline acetyltransferase and muscarinic cholinergic receptors: application in aged-rat brain.

作者信息

Haba K, Ogawa N, Kawata M, Mori A

机构信息

Department of Neurochemistry, Okayama University Medical School, Japan.

出版信息

Neurochem Res. 1988 Oct;13(10):951-5. doi: 10.1007/BF00970767.

DOI:10.1007/BF00970767
PMID:3216953
Abstract

We have devised a method for the parallel determination of choline acetyltransferase (CAT) and muscarinic cholinergic receptor (mCh-R) in the same brain tissue. The method for CAT activity determination is more rapid, simplified, stable, and economical than the usual Fonnum's method. With our method, age-associated changes in CAT activity and mCh-R levels were examined. Although CAT activity hardly changed with age except in a few areas, mCh-R binding of aged-rats was markedly reduced in all areas. These results suggest that the change in mCh-R represents an age-associated biochemical change in the brain and that determination of CAT activity is not sufficient for the study of age-associated changes in the brain cholinergic system.

摘要

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