Department of Microbiology, Faculty of Veterinary Medicine, Ataturk University, 25240 Erzurum, Turkey.
Department of Medical Pharmacology, Faculty of Medicine, Seyh Edebali University, 11000 Bilecik, Turkey.
Mol Med Rep. 2022 Sep;26(3). doi: 10.3892/mmr.2022.12809. Epub 2022 Aug 3.
Methicillin‑resistant (MRSA) infections are usually found in hospital settings and, frequently, in patients with open wounds. One of the most critical virulence factors affecting the severity and recurrence of infections is the biofilm; increasing antibiotic resistance due to biofilm formation has led to the search for alternative compounds to antibiotics. The present study aimed to use boric acid and potassium metaborate against MRSA infection in a fibroblast wound model. For this purpose, a two‑part experiment was designed: First, MRSA strains were used for the test, and both boric acid and potassium metaborate were prepared in microdilution. In the second step, an MRSA wound model was prepared using a fibroblast culture, and treatments with boric acid and potassium metaborate were applied for 24 h. For the evaluation of the effects of treatment, cell viability assay (MTT assay), analysis of redox stress parameters, including total oxidant status and total antioxidant capacity analyses, lactate dehydrogenase analysis and immunohistochemical staining were performed. In addition, IL‑1β and IL‑10 gene expression levels were assayed. According to the results, potassium metaborate was more effective and exhibited a lower toxicity to fibroblast cells compared to boric acid; moreover, potassium metaborate decreased the level of prooxidant species and increased the antioxidant status more effectively than boric acid. The IL‑1β level in the bacteria group was high; however, boric acid and potassium metaborate significantly decreased the expression levels of inflammatory markers, exhibiting the potential to improve the resolution of the lesion. On the whole, the findings of the present study suggest that boric acid and potassium metaborate may be effective on the tested microorganisms.
耐甲氧西林金黄色葡萄球菌（MRSA）感染通常在医院环境中发现，并且经常在有开放性伤口的患者中发现。影响感染严重程度和复发的最重要毒力因子之一是生物膜；由于生物膜的形成导致抗生素耐药性增加，因此人们一直在寻找抗生素的替代化合物。本研究旨在使用硼酸和偏硼酸钾来治疗成纤维细胞伤口模型中的 MRSA 感染。为此，设计了两部分实验：首先，使用 MRSA 菌株进行测试，并且在微量稀释法中制备了硼酸和偏硼酸钾。在第二步中，使用成纤维细胞培养物制备了 MRSA 伤口模型，并应用硼酸和偏硼酸钾进行了 24 小时的治疗。为了评估治疗效果，进行了细胞活力测定（MTT 测定）、氧化应激参数分析，包括总氧化剂状态和总抗氧化能力分析、乳酸脱氢酶分析和免疫组织化学染色。此外，还测定了 IL-1β 和 IL-10 基因表达水平。根据结果，与硼酸相比，偏硼酸钾对成纤维细胞更有效，毒性更低；此外，偏硼酸钾降低了促氧化剂的水平，并且比硼酸更有效地增加了抗氧化状态。细菌组的 IL-1β 水平较高；但是，硼酸和偏硼酸钾显著降低了炎症标志物的表达水平，表明其有改善病变缓解的潜力。总的来说，本研究的结果表明硼酸和偏硼酸钾可能对测试的微生物有效。
