组织切片的链霉蛋白酶预处理可提高未标记抗体-酶（PAP）技术的敏感性。
Pronase pretreatment of tissue sections enhances sensitivity of the unlabelled antibody-enzyme (PAP) technique.
作者信息
Denk H, Radaszkiewicz T, Weirich E
出版信息
J Immunol Methods. 1977;15(2):163-7. doi: 10.1016/0022-1759(77)90027-8.
PMID:321699
Abstract
Treatment of dewaxed sections of formalin-fixed paraffin embedded material with 0.1% pronase for 15 min at 37 degrees C prior to the performance of the unlabelled antibody-enzyme (PAP) procedure greatly enhanced its sensitivity. This was shown by detection of immunoglobulin within plasma cells in chronic gastritis specimens.
摘要
在进行未标记抗体-酶（PAP）程序之前，将福尔马林固定石蜡包埋材料的脱蜡切片在37℃下用0.1%链霉蛋白酶处理15分钟，可大大提高其敏感性。这在慢性胃炎标本浆细胞内免疫球蛋白的检测中得到了证实。
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