链霉蛋白酶的使用提高了PAP法检测胞浆内免疫球蛋白的敏感性。
The use of pronase enhances sensitivity of the PAP method in the detection of intracytoplasmic immunoglobulins.
作者信息
Pileri S, Serra L, Martinelli G
出版信息
Basic Appl Histochem. 1980;24(3):203-7.
PMID:6160845
Abstract
Application of immunocytochemistry to formalin-fixed, paraffin-embedded material tends to provide capricious results, even when employing the unlabelled antibody peroxidase-antiperoxidase (PAP) method. This study shows that treatment of tissue sections with pronase, a protease isolated from a strain of Streptomyces griseus, prior to performance of the PAP procedure greatly enhances its sensitivity. The optimum conditions for use of this enzyme in detecting intracellular immunoglobulins are given.
摘要
即使采用未标记抗体过氧化物酶-抗过氧化物酶（PAP）方法，将免疫细胞化学应用于福尔马林固定、石蜡包埋的材料时，往往会得到不稳定的结果。本研究表明，在进行PAP程序之前，用链霉菌蛋白酶（一种从灰色链霉菌菌株中分离出的蛋白酶）处理组织切片，可大大提高其敏感性。文中给出了在检测细胞内免疫球蛋白时使用该酶的最佳条件。
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