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未标记抗体-酶（PAP）法的背景染色及敏感性。与使用福尔马林固定石蜡包埋材料的过氧化物酶标记抗体夹心法的比较。

Background staining and sensitivity of the unlabelled antibody-enzyme (PAP) method. Comparison with the peroxidase labelled antibody sandwich method using formalin fixed paraffin embedded material.

作者信息

Burns J

出版信息

Histochemistry. 1975 Jun 5;43(3):291-4. doi: 10.1007/BF00499711.

DOI:10.1007/BF00499711
PMID:50308
Abstract

The PAP procedure was compared with the peroxidase labelled antibody sandwich method and was found to be at least 20 times more sensitive. Background staining was reduced by the addition of normal swine serum to all the immune sera or by pretreating sections with it at the beginning of either method. The PAP procedure could be effectively reduced to a period of 1 hour or less.

摘要

将PAP法与过氧化物酶标记抗体夹心法进行比较，发现前者的敏感性至少高20倍。通过在所有免疫血清中加入正常猪血清，或者在任何一种方法开始时用其预处理切片，可减少背景染色。PAP法可有效地缩短至1小时或更短时间。

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1
Background staining and sensitivity of the unlabelled antibody-enzyme (PAP) method. Comparison with the peroxidase labelled antibody sandwich method using formalin fixed paraffin embedded material.未标记抗体-酶（PAP）法的背景染色及敏感性。与使用福尔马林固定石蜡包埋材料的过氧化物酶标记抗体夹心法的比较。
Histochemistry. 1975 Jun 5;43(3):291-4. doi: 10.1007/BF00499711.
2
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本文引用的文献

1
The role of cathepsin D in the pathogenesis of tuberculosis. A histochemical study employing unlabeled antibodies and the peroxidase-antiperoxidase complex.组织蛋白酶D在结核病发病机制中的作用。一项采用未标记抗体和过氧化物酶-抗过氧化物酶复合物的组织化学研究。
Am J Pathol. 1974 Jan;74(1):1-17.
2
Intracellular immunoglobulins. A comparative study on three standard tissue processing methods using horseradish peroxidase and fluorochrome conjugates.细胞内免疫球蛋白。使用辣根过氧化物酶和荧光染料偶联物对三种标准组织处理方法的比较研究。
J Clin Pathol. 1974 Jul;27(7):548-57. doi: 10.1136/jcp.27.7.548.
3
An immunoperoxidase technique for the identification of gastrin-producing cells.
慢性腮腺炎及腮腺恶性淋巴瘤中含免疫球蛋白的浆细胞。免疫细胞化学观察频率及定位的比较。
Virchows Arch A Pathol Anat Histol. 1980;389(1):79-92. doi: 10.1007/BF00428669.
4
Cleavability of hemagglutinin determines spread of avian influenza viruses in the chorioallantoic membrane of chicken embryo.血凝素的可切割性决定禽流感病毒在鸡胚尿囊膜中的传播。
Arch Virol. 1980;65(2):123-33. doi: 10.1007/BF01317323.
5
Malignant histiocytosis. Immunohistochemical characterization on paraffin embedded tissue.恶性组织细胞增多症。石蜡包埋组织的免疫组织化学特征
Virchows Arch A Pathol Anat Histol. 1980;385(2):233-46. doi: 10.1007/BF00427407.
6
Nephrotic syndrome and renal insufficiency in association with amyloidosis: a correlation between structure and function.肾病综合征及肾功能不全与淀粉样变性的关联：结构与功能之间的相关性
Klin Wochenschr. 1981 Jan 15;59(2):75-82. doi: 10.1007/BF01477286.
7
Distribution of plasma cells in normal rectal mucosa.正常直肠黏膜中浆细胞的分布。
J Clin Pathol. 1982 Aug;35(8):820-3. doi: 10.1136/jcp.35.8.820.
8
Apparent monoclonal origin of lymphocytes and plasma cells infiltrating ocular adnexal amyloid deposits: report of 2 cases.浸润眼附属器淀粉样沉积物的淋巴细胞和浆细胞明显呈单克隆起源：2例报告
Br J Ophthalmol. 1982 Sep;66(9):600-6. doi: 10.1136/bjo.66.9.600.
9
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Virchows Arch A Pathol Anat Histol. 1981;394(1-2):109-18. doi: 10.1007/BF00431669.
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4
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Histochemistry. 1974;40(4):343-8. doi: 10.1007/BF00495041.
5
Letter: Destruction of endogenous peroxidase activity in order to locate cellular antigens by peroxidase-labeled antibodies.信函：通过过氧化物酶标记抗体定位细胞抗原时内源性过氧化物酶活性的破坏
J Histochem Cytochem. 1974 Jan;22(1):51-4. doi: 10.1177/22.1.51.
6
Localization of lysozyme in normal rat tissues by an immunoperoxidase method.采用免疫过氧化物酶法对正常大鼠组织中的溶菌酶进行定位。
J Histochem Cytochem. 1974 Mar;22(3):139-46. doi: 10.1177/22.3.139.
7
Letter: The use of formalin fixation in an improved method for immunofluorescence histochemical investigation of neurophysin in the hypothalamus.信函：在下丘脑神经垂体素免疫荧光组织化学研究的一种改进方法中使用福尔马林固定。
J Histochem Cytochem. 1974 Feb;22(2):128-30. doi: 10.1177/22.2.128.
8
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J Clin Pathol. 1974 Jan;27(1):14-20. doi: 10.1136/jcp.27.1.14.
9
Inhibition of erythrocyte pseudoperoxidase activity by treatment with hydrogen peroxide following methanol.甲醇处理后用过氧化氢抑制红细胞假过氧化物酶活性。
J Histochem Cytochem. 1972 Oct;20(10):829-31. doi: 10.1177/20.10.829.