CRISPR-Cas12b 可实现高效的植物基因组编辑。

CRISPR-Cas12b enables efficient plant genome engineering.

机构信息

Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA.

Centre of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.

出版信息

Nat Plants. 2020 Mar;6(3):202-208. doi: 10.1038/s41477-020-0614-6. Epub 2020 Mar 9.

DOI:10.1038/s41477-020-0614-6

PMID:32170285

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12b is a newly emerged genome engineering system. Here, we compared Cas12b from Alicyclobacillus acidoterrestris (Aac), Alicyclobacillus acidiphilus (Aa), Bacillus thermoamylovorans (Bth) and Bacillus hisashii (Bh) for genome engineering in rice, an important crop. We found AaCas12b was more efficient than AacCas12b and BthCas12b for targeted mutagenesis, which was further demonstrated in multiplexed genome editing. We also engineered the Cas12b systems for targeted transcriptional repression and activation. Our work establishes Cas12b as the third promising CRISPR system, after Cas9 and Cas12a, for plant genome engineering.

摘要

成簇规律间隔短回文重复序列 (CRISPR)-Cas12b 是一种新兴的基因组工程系统。在这里，我们比较了来自酸土嗜酸杆菌（Aac）、嗜酸热脂芽孢杆菌（Aa）、解淀粉芽孢杆菌（Bth）和地衣芽孢杆菌（Bh）的 Cas12b 在水稻中的基因组工程应用，水稻是一种重要的作物。我们发现 AaCas12b 比 AacCas12b 和 BthCas12b 更有效地进行靶向诱变，这在多重基因组编辑中得到了进一步证明。我们还对 Cas12b 系统进行了靶向转录抑制和激活的工程设计。我们的工作确立了 Cas12b 作为继 Cas9 和 Cas12a 之后，在植物基因组工程方面的第三个有前途的 CRISPR 系统。