School of Applied Biosciences, Kyungpook National University, Daegu, 37224, Republic of Korea.
Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeollabuk-do, 56212, Republic of Korea.
Curr Microbiol. 2020 Aug;77(8):1902-1908. doi: 10.1007/s00284-020-01940-5. Epub 2020 Mar 13.
A Gram-negative, aerobic, non-motile, rod-shaped, floc-forming, and non-spore-forming bacterium, designated as NLF-7-7, was isolated from the biofilm of a sample collected from a livestock wastewater treatment plant in Nonsan, Republic of Korea. Strain NLF-7-7, forms a visible floc and grows in the flocculated state. Cells of strain NLF-7-7 grew optimally at pH 6.5 and 30 °C and in the presence of 0.5% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NLF-7-7 belonged to the family Comamonadaceae, and was most closely related to Comamonas badia DSM 17552 (95.8% similarity) and Comamonas nitrativorans 23310 (94.0% similarity). The phylogenetic and phenotypic data indicate strain NLF-7-7 is clearly distinguished from the Comamonas lineage. The major cellular fatty acids were C 3OH, C, and summed feature 3 (C ω6c/C ω7c). The respiratory quinone was Q-8. The polar lipids were composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and an unidentified aminolipid. The DNA G+C content of strain NLF-7-7 was 68.0 mol%. Based on the phenotypic, chemotaxonomic, and phylogenetic properties, strain NLF-7-7 represents a novel species of the genus Comamonas, for which the name Comamonas flocculans sp. nov. is proposed. The type strain is C. flocculans NLF-7-7 (=KCTC 62943). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Comamonas flocculans NLF-7-7 is MN527436. The whole-genome shotgun BioProject Number is PRJNA555370 with the Accession Number CP042344.
一株革兰氏阴性、需氧、不运动、杆状、絮状形成、不产孢的细菌，被命名为 NLF-7-7，是从韩国论山市一家家畜废水处理厂的生物膜样本中分离出来的。菌株 NLF-7-7 形成可见的絮状物，并在絮状状态下生长。菌株 NLF-7-7 的最佳生长条件为 pH 6.5、30°C 和 0.5%（w/v）NaCl。基于 16S rRNA 基因序列的系统发育分析表明，菌株 NLF-7-7 属于科科蒙纳氏菌科，与 Comamonas badia DSM 17552（相似度 95.8%）和 Comamonas nitrativorans 23310（相似度 94.0%）最为密切相关。系统发育和表型数据表明，菌株 NLF-7-7 与科蒙纳氏菌属明显不同。主要的细胞脂肪酸为 C 3OH、C 和总和特征 3（C ω6c/C ω7c）。呼吸醌为 Q-8。极性脂由双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和一种未鉴定的胺脂组成。菌株 NLF-7-7 的 DNA G+C 含量为 68.0 mol%。基于表型、化学分类和系统发育特性，菌株 NLF-7-7 代表科蒙纳氏菌属的一个新种，建议将其命名为 Comamonas flocculans sp. nov. 。模式株为 C. flocculans NLF-7-7（=KCTC 62943）。Comamonas flocculans NLF-7-7 的 16S rRNA 基因序列的 GenBank/EMBL/DDBJ 登录号为 MN527436。全基因组鸟枪法生物项目编号为 PRJNA555370，登录号为 CP042344。
