ICAR- Central Marine Fisheries Research Institute, Ernakulam North P.O., Kochi, Kerala, 682018, India.
Biochem Genet. 2020 Jun;58(3):430-451. doi: 10.1007/s10528-020-09956-z. Epub 2020 Mar 13.
Mitochondrial genomes are valuable for making inferences regarding population genetics, phylogenetics and recently in understanding adaptive variation in OXPHOS genes. Ribbon fish, Trichiurus lepturus is distributed in the Indian Ocean and contributes to a major share of pelagic fish landings. Considering the importance, we characterized the complete mitogenome of large head hairtail T. lepturus from Indian waters by PCR amplification and sequencing. The entire mitogenome is 16,840 bp in length, with 13 protein coding genes, 2 subunit ribosomal RNA genes, and 1 control region similar to other vertebrates. Genome contains only 21 tRNAs (tRNA is absent) as against 22 tRNAs reported in other vertebrates. The putative origin of L-strand replication (O) was located in a cluster of five tRNA genes (WANCY region) and may not be functional due to the variation of conserved motif (5'-GCCGG-3') and loop. These two unusual features are common for Trichiurus mitogenomes. Phylogenetic analysis supported the monophyly of Trichiuridae. This study will provide information for further studies on taxonomy, phylogeny and patterns of adaptive evolution in the mitogenome of Trichiurus species.
线粒体基因组对于推断种群遗传学、系统发生学以及最近对氧化磷酸化（OXPHOS）基因适应性变异的理解非常有价值。带鱼分布于印度洋，是远洋鱼类捕捞的主要品种之一。考虑到其重要性，我们通过 PCR 扩增和测序，对来自印度海域的大型带鱼 Trichiurus lepturus 的完整线粒体基因组进行了特征描述。该完整线粒体基因组全长 16840bp，包含 13 个蛋白编码基因、2 个亚单位核糖体 RNA 基因和 1 个与其他脊椎动物相似的控制区。与其他脊椎动物报告的 22 个 tRNA 不同，基因组仅包含 21 个 tRNA（缺失 tRNA）。假定的 L 链复制起点（O）位于 5 个 tRNA 基因（WANCY 区）的簇中，由于保守基序（5'-GCCGG-3'）和环的变化，可能没有功能。这两个不寻常的特征在 Trichiurus 线粒体基因组中很常见。系统发育分析支持长尾带鱼科的单系性。本研究将为进一步研究长尾带鱼属的分类学、系统发生和适应进化模式提供信息。
