镰状细胞病患者脂质过氧化与一氧化氮代谢物、微量元素和抗氧化酶的相关性。
Correlation of lipid peroxidation and nitric oxide metabolites, trace elements, and antioxidant enzymes in patients with sickle cell disease.
机构信息
Department of Physiology, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana.
Department of Anaesthesia, School of Medicine and Dentistry, University of Ghana, Accra, Ghana.
出版信息
J Clin Lab Anal. 2020 Jul;34(7):e23294. doi: 10.1002/jcla.23294. Epub 2020 Mar 13.
BACKGROUND
Lipid peroxidation plays a very important role in sickle cell pathophysiology. The formation of malondialdehyde (MDA) in patients with sickle cell disease (SCD) may lead to endothelial dysfunction. Nitric oxide (NO) is a known vasodilator which plays a role in endothelial function. The current study determined the association between MDA and NO metabolites (NOx), trace elements, and antioxidant enzymes (SOD and CAT) in patients with SCD. The ratio of MDA/NOx was also determined as an index of oxidative stress in the study groups.
METHODS
This was a cross-sectional study involving 90 patients with SCD and 50 "healthy" controls. Blood samples (n = 140) were collected from the study groups. The plasma, sera, and red cells were kept at -20°C for biochemical analyses. Hemoglobin (Hb) and NOx levels were determined in the plasma using Labsystem Multiskan MS and Griess reagent system, respectively. Super oxide dismutase (SOD) and catalase (CAT) levels were determined in the red cells using assay kits from Cayman chemicals. Lipid peroxidation biomarker MDA was determined in the sera using the TBARS assay. Levels of iron (Fe), copper (Cu), and zinc (Zn) were also determined in the sera using Variant 240FS. MDA and NOx ratio was computed for the study groups and compared.
RESULTS
Levels of Hb, NOx, SOD, CAT, and Zn were significantly lower in the patients with SCD (P < .001). MDA, Fe, and MDA/ NOx ratio were, however, significantly higher in the patients with SCD (P < .001). There was no significant correlation between MDA and NOx, SOD, CAT, Fe, and Zn in the study groups. MDA, however, correlated positively and significantly with Cu in the HbSS patients with vaso-occlusive crises (VOC). Gender did not affect the levels of oxidative stress markers.
CONCLUSIONS
Findings from this study suggest a link between lipid peroxidation and Cu in HbSS patients with VOC. Increased MDA/NOx ratio may contribute to sickle cell pathophysiology by promoting oxidative stress.
背景
脂质过氧化在镰状细胞病理生理学中起着非常重要的作用。镰状细胞病（SCD）患者丙二醛（MDA）的形成可能导致内皮功能障碍。一氧化氮（NO）是一种已知的血管扩张剂，在内皮功能中起作用。本研究旨在确定 SCD 患者 MDA 与 NO 代谢物（NOx）、微量元素和抗氧化酶（SOD 和 CAT）之间的关系。研究组还测定了 MDA/NOx 比值作为氧化应激的指标。
方法
这是一项横断面研究，纳入了 90 名 SCD 患者和 50 名“健康”对照者。从研究组采集了 140 份血样。血浆、血清和红细胞在-20°C 下保存，用于生化分析。使用 Labsystem Multiskan MS 和 Griess 试剂系统分别测定血浆中的血红蛋白（Hb）和 NOx 水平。使用 Cayman 化学试剂盒测定红细胞中超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的水平。使用 TBARS 测定法测定血清中的脂质过氧化生物标志物 MDA。使用 Variant 240FS 测定血清中的铁（Fe）、铜（Cu）和锌（Zn）水平。计算了研究组的 MDA 和 NOx 比值并进行了比较。
结果
SCD 患者的 Hb、NOx、SOD、CAT 和 Zn 水平显著降低（P<0.001）。然而，SCD 患者的 MDA、Fe 和 MDA/NOx 比值显著升高（P<0.001）。研究组中 MDA 与 NOx、SOD、CAT、Fe 和 Zn 之间无显著相关性。然而，HbSS 伴有血管阻塞危象（VOC）的患者中 MDA 与 Cu 呈正相关且显著相关。性别对氧化应激标志物水平无影响。
结论
本研究结果表明，HbSS 伴有 VOC 的患者中脂质过氧化与 Cu 之间存在关联。增加的 MDA/NOx 比值可能通过促进氧化应激来促进镰状细胞病理生理学。
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