Okamoto Camila Basilio, Bussadori Sandra Kalil, Prates Renato Araujo, da Mota Ana Carolina Costa, Tempestini Horliana Anna Carolina Ratto, Fernandes Kristianne Porta Santos, Motta Lara Jansiski
Program of Biophotonics Applied to the Health Sciences, Nove de Julho University, São Paulo, Brazil.
Program of Biophotonics Applied to the Health Sciences, Nove de Julho University, São Paulo, Brazil; Biophotonics Applied to the Health Sciences, Nove de Julho University, Rua Vergueiro, 235/249 - Liberdade, 01504-000, São Paulo, SP, Brazil.
Photodiagnosis Photodyn Ther. 2020 Jun;30:101732. doi: 10.1016/j.pdpdt.2020.101732. Epub 2020 Apr 7.
This study aimed to evaluate the reduction in bacterial load following conventional endodontic treatment with and without antimicrobial photodynamic therapy (a-PDT) in primary teeth.
Thirty primary anterior teeth with a diagnosis of pulp necrosis were selected. Patients were randomly allocated to two groups as follows: Group I, patients undergoing conventional root canal therapy (n = 15) and Group II, patients undergoing conventional root canal therapy combined with antimicrobial PDT (n = 15). For PDT, methylene blue, at a concentration of 0.005 %, was used as the photosensitizing agent, which was applied to the interior of the canal with a sterile paper cone for 3 min, followed by the administration of laser light for 40 s (wavelength: 660 nm, energy density: 4 J/cm², power: 100 mW), delivered in direct contact at the entrance to the root canal. Two microbiological samples of the intra-canal content were taken (one before and one immediately after treatment in both groups) using paper cones. Clinical follow-up involved the investigation of fistulas and mobility and was performed 1- and 3 months after treatment. Data were statistically analyzed.
The reduction in bacterial load was 93 % in Group I and 99 % in Group II, with no statistically significant difference.
Conventional treatment combined with antimicrobial PDT with parameters used in this study proved effective but presented equal efficacious capability to conventional endodontic treatment alone.
The use of PDT has been studied in endodontic treatment protocols in permanent teeth. However, clinical trials in deciduous teeth are necessary for establishing the effectiveness and parameters of this application. The present study analyzes the results of PDT in the endodontic treatment of deciduous teeth.
本研究旨在评估乳牙在接受和未接受抗菌光动力疗法（a-PDT）的传统牙髓治疗后细菌载量的降低情况。
选取30颗诊断为牙髓坏死的乳前牙。患者被随机分为两组：第一组，接受传统根管治疗的患者（n = 15）；第二组，接受传统根管治疗联合抗菌光动力疗法的患者（n = 15）。对于光动力疗法，使用浓度为0.005%的亚甲蓝作为光敏剂，用无菌纸尖将其应用于根管内部3分钟，然后给予激光照射40秒（波长：660 nm，能量密度：4 J/cm²，功率：100 mW），直接接触根管入口处进行照射。使用纸尖采集两组根管内容物的两份微生物样本（一组在治疗前采集，另一组在治疗后立即采集）。临床随访包括对瘘管和牙齿松动度的检查，在治疗后1个月和3个月进行。对数据进行统计学分析。
第一组细菌载量降低了93%，第二组降低了99%，两组之间无统计学显著差异。
本研究中使用的参数下，传统治疗联合抗菌光动力疗法被证明是有效的，但与单独的传统牙髓治疗具有同等的疗效能力。
光动力疗法在恒牙牙髓治疗方案中的应用已有研究。然而，对于乳牙的临床试验对于确定该应用的有效性和参数是必要的。本研究分析了光动力疗法在乳牙牙髓治疗中的结果。
