Engineering Research Centre of Bioprocess of Ministry of Education, Hefei University of Technology, No 193 Tunxi Road, Hefei 230009, People's Republic of China; School of Food and Biological Engineering, Hefei University of Technology, No 193 Tunxi Road, Hefei 230009, People's Republic of China.
Engineering Research Centre of Bioprocess of Ministry of Education, Hefei University of Technology, No 193 Tunxi Road, Hefei 230009, People's Republic of China; School of Food and Biological Engineering, Hefei University of Technology, No 193 Tunxi Road, Hefei 230009, People's Republic of China; Key Laboratory of Metabolism and Regulation for Major Disease of Anhui Higher Education Institues, Hefei University of Technology, No 193 Tunxi Road, Hefei 230009, People's Republic of China.
Carbohydr Polym. 2020 May 15;236:116009. doi: 10.1016/j.carbpol.2020.116009. Epub 2020 Feb 17.
In this study, whey protein isolate (WPI)-quercetin (Que)-lotus root amylopectin (LRA) hydrogels (WPI-QUE-LRA) was developed to improve the solubility, stability and bioavailability of quercetin. Results showed that the favorable WPI-QUE-LRA was formed using WPI and LRA in the ratio of 1:2 at pH 7.0. Under this condition, the average size, polydispersity index, zeta potential of the WPI-QUE-LRA was 179.5 nm, 0.271, -18.6 mV, respectively. The analysis of transmission electron microscopy, fourier transform infrared spectroscopy and X-ray diffractometer revealed that the quercetin was successfully encapsulated in WPI-LRA, giving a high encapsulation efficiency of 92.4 %. Moreover, the WPI-LRA could significantly improve the storage stability and photochemical stability of quercetin. The in vitro and in vivo experiments showed that LRA-coated WPI hydrogel can enable quercetin to be stable in stomach and be effectively released in small intestine, leading to the enhancement of the bioavailability of quercetin.
在这项研究中，开发了乳清蛋白分离物（WPI）-槲皮素（Que）-莲藕直链淀粉（LRA）水凝胶（WPI-QUE-LRA），以提高槲皮素的溶解度、稳定性和生物利用度。结果表明，在 pH 值为 7.0 时，使用 WPI 和 LRA 的比例为 1:2 可以形成有利的 WPI-QUE-LRA。在这种条件下，WPI-QUE-LRA 的平均粒径、多分散指数和 Zeta 电位分别为 179.5nm、0.271 和-18.6mV。透射电子显微镜、傅里叶变换红外光谱和 X 射线衍射仪的分析表明，槲皮素已成功包封在 WPI-LRA 中，包封效率高达 92.4%。此外，WPI-LRA 可以显著提高槲皮素的储存稳定性和光化学稳定性。体外和体内实验表明，LRA 包覆的 WPI 水凝胶可以使槲皮素在胃中稳定，并在小肠中有效释放，从而提高槲皮素的生物利用度。
