Engineering Research Centre of Bioprocess of Ministry of Education, School of Food and Biological Engineering, Hefei University of Technology, No 193 Tunxi Road, Hefei 230009, People's Republic of China; Anhui Engineering Laboratory for Agro-products Processing, Food Processing Research Institute, College of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036, People's Republic of China.
Engineering Research Centre of Bioprocess of Ministry of Education, School of Food and Biological Engineering, Hefei University of Technology, No 193 Tunxi Road, Hefei 230009, People's Republic of China.
Food Chem. 2022 Sep 1;387:132924. doi: 10.1016/j.foodchem.2022.132924. Epub 2022 Apr 9.
To improve the water solubility, stability and bioavailability of quercetin, the quercetin (Que)-quinoa protein (QP)-lotus root amylopectin (LRA) nanomicelles (Que-QP-LRA) were constructed via self-assembly in the present study. Results showed that a uniform and stable Que-QP-LRA nanomicelles was formed when the mass ratio of Que/QP/LRA was 2.5:6:24. Under this condition, the particle size, polydispersity index and zeta potential of the nanomicelles were 157.3 nm, 0.289 and -16.7 mV, respectively. Transmission electron microscopy exhibited that the Que-QP-LRA nanomicelles have a core-shell structure. The analysis of molecular interaction indicated that hydrogen bonding and hydrophobic interaction were the main driving forces to maintain stable structure of Que-QP-LRA nanomicelles. Additionally, the in vitro simulated digestion experiments suggested that Que-QP-LRA nanomicelles can enhance the stability of quercetin in the stomach and enable it to be sustained release in the intestine. These results suggested that Que-QP-LRA nanomicelles were beneficial for improving the bioavailability of quercetin.
为提高槲皮素的水溶性、稳定性和生物利用度，本研究通过自组装构建了槲皮素（Que）-藜麦蛋白（QP）-莲藕直链淀粉（LRA）纳米胶束（Que-QP-LRA）。结果表明，当 Que/QP/LRA 的质量比为 2.5:6:24 时，可形成均匀稳定的 Que-QP-LRA 纳米胶束。在此条件下，纳米胶束的粒径、多分散指数和 Zeta 电位分别为 157.3nm、0.289 和-16.7mV。透射电子显微镜显示，Que-QP-LRA 纳米胶束具有核壳结构。分子相互作用分析表明，氢键和疏水相互作用是维持 Que-QP-LRA 纳米胶束稳定结构的主要驱动力。此外，体外模拟消化实验表明，Que-QP-LRA 纳米胶束可以提高槲皮素在胃中的稳定性，并使其在肠道中持续释放。这些结果表明，Que-QP-LRA 纳米胶束有利于提高槲皮素的生物利用度。
