Laboratory of Fruit Tree Biotechnology, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Gene. 2020 Jun 5;742:144584. doi: 10.1016/j.gene.2020.144584. Epub 2020 Mar 13.
Sugars are considered as an essential signaling molecule for fruit growth and development, which plays a key role in fruit quality. Up to now, the mechanism controlling sugar metabolism and transport in apricot is unclear. Therefore, in the present study, we measured sugar contents at six different stages of fruit development and ripening, and significant variations were observed throughout these stages. The concentration of glucose and fructose first decreased then increased, sucrose concentration first increased then decreased, while the concentration of sorbitol gradually decreased from growth to maturity. Furthermore, thirty sugar transporter genes related to sucrose synthesis and transport were identified and categorized into different subfamilies based on the phylogenetic analysis. The result of cis-regulatory components showed that under different plant hormones, biotic and abiotic stresses, few elements could be regulated. The correlation analysis showed a higher relationship between ParSuSy5, ParSuSy6, ParSuSy7, and ParFK1 genes and sugar contents, indicating that these genes might have a key role in sugar accumulation and fruit quality. In general, these findings will provide a deep understanding of genomic information and expression profiles of sugar transporter genes, which will contribute toward improvement in fruit quality of apricot.
糖被认为是果实生长和发育的重要信号分子，在果实品质中起着关键作用。迄今为止，控制杏果实糖代谢和运输的机制尚不清楚。因此，在本研究中，我们测量了果实发育和成熟的六个不同阶段的糖含量，在整个阶段都观察到了显著的变化。葡萄糖和果糖的浓度先降低后升高，蔗糖浓度先升高后降低，而山梨醇的浓度则从生长到成熟逐渐降低。此外，鉴定了 30 个与蔗糖合成和运输相关的糖转运蛋白基因，并根据系统发育分析将它们分为不同的亚家族。顺式调控元件的结果表明，在不同的植物激素、生物和非生物胁迫下，只有少数元件可以被调控。相关性分析表明，ParSuSy5、ParSuSy6、ParSuSy7 和 ParFK1 基因与糖含量的关系更高，这表明这些基因可能在糖积累和果实品质中起关键作用。总的来说，这些发现将为糖转运蛋白基因的基因组信息和表达谱提供深入的了解，这将有助于提高杏果实的品质。
