Xi Wanpeng, Zheng Huiwen, Zhang Qiuyun, Li Wenhui
College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China.
Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China.
Int J Mol Sci. 2016 Jun 24;17(7):998. doi: 10.3390/ijms17070998.
Sugars, organic acids and volatiles of apricot were determined by HPLC and GC-MS during fruit development and ripening, and the key taste and aroma components were identified by integrating flavor compound contents with consumers' evaluation. Sucrose and glucose were the major sugars in apricot fruit. The contents of all sugars increased rapidly, and the accumulation pattern of sugars converted from glucose-predominated to sucrose-predominated during fruit development and ripening. Sucrose synthase (SS), sorbitol oxidase (SO) and sorbitol dehydrogenase (SDH) are under tight developmental control and they might play important roles in sugar accumulation. Almost all organic acids identified increased during early development and then decrease rapidly. During early development, fruit mainly accumulated quinate and malate, with the increase of citrate after maturation, and quinate, malate and citrate were the predominant organic acids at the ripening stage. The odor activity values (OAV) of aroma volatiles showed that 18 aroma compounds were the characteristic components of apricot fruit. Aldehydes and terpenes decreased significantly during the whole development period, whereas lactones and apocarotenoids significantly increased with fruit ripening. The partial least squares regression (PLSR) results revealed that β-ionone, γ-decalactone, sucrose and citrate are the key characteristic flavor factors contributing to consumer acceptance. Carotenoid cleavage dioxygenases (CCD) may be involved in β-ionone formation in apricot fruit.
在果实发育和成熟过程中,采用高效液相色谱法(HPLC)和气相色谱-质谱联用仪(GC-MS)测定了杏的糖类、有机酸和挥发性成分,并通过整合风味化合物含量与消费者评价来鉴定关键的风味和香气成分。蔗糖和葡萄糖是杏果实中的主要糖类。所有糖类含量迅速增加,在果实发育和成熟过程中,糖类积累模式从以葡萄糖为主转变为以蔗糖为主。蔗糖合酶(SS)、山梨醇氧化酶(SO)和山梨醇脱氢酶(SDH)受到严格的发育调控,它们可能在糖类积累中发挥重要作用。几乎所有鉴定出的有机酸在发育早期增加,然后迅速下降。在发育早期,果实主要积累奎尼酸和苹果酸,成熟后柠檬酸增加,在成熟阶段,奎尼酸、苹果酸和柠檬酸是主要的有机酸。香气挥发物的气味活性值(OAV)表明,18种香气化合物是杏果实的特征成分。醛类和萜类在整个发育过程中显著下降,而内酯类和类胡萝卜素降解产物随着果实成熟显著增加。偏最小二乘回归(PLSR)结果表明,β-紫罗兰酮、γ-癸内酯、蔗糖和柠檬酸是影响消费者接受度的关键特征风味因子。类胡萝卜素裂解双加氧酶(CCD)可能参与了杏果实中β-紫罗兰酮的形成。