Shabandoust Hajar, Sharifi Iraj, Raiesi Omid, Getso Muhammad Ibrahim, Dezaki Saedi Ebrahim, Afgar Ali, Shirvani Giti, Salarkia Ehsan, Babaei Zahra
1Department of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran.
2Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.
J Parasit Dis. 2020 Mar;44(1):40-48. doi: 10.1007/s12639-019-01159-7. Epub 2019 Sep 26.
, an obligate intracellular parasite is eliminated by a strong Th-1 host response. As Vitamin D metabolism and its receptor activity are important factors in human native immune system against some microorganisms, we hypothesized that VDR gene polymorphisms and concentration of Vitamin D might have effect on incidence of cutaneous leishmaniasis. The aim of this study was to investigate the association between VDR gene polymorphism and/or the serum vitamin D level and leishmaniasis in the infected patients in comparison to the healthy individuals. In this case-control study, the BsmI, FokI and Taq1 polymorphisms in the VDR gene and serum levels of vitamin D were studied in Iranian infected with (n = 50) and healthy controls using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and Electrochemiluminescence methods respectively. Data were statistically analyzed using SPSS software, Chi square and ANOVA tests. The results of this study showed that despite the relatively higher frequency of BsmI-BB, FokI-FF and TaqI-Tt than Non BsmI-BB, Non FokI-FF and Non TaqI-Tt in the patients compared with the healthy individuals, the differences were not statistically significant ( > 0.05). Based on our findings, the relationship between the VDR polymorphism, the serum concentration of 25-hydroxyvitamin D and the susceptibility to infection, remains unclear requiring further in-depth studies. However, for better interpretation, it is necessary to consider factors such as the size of the sample examined and the other alleles of VDR, including ApaI.
一种专性细胞内寄生虫可通过强烈的Th-1宿主反应被清除。由于维生素D代谢及其受体活性是人类天然免疫系统抵御某些微生物的重要因素，我们推测维生素D受体（VDR）基因多态性和维生素D浓度可能对皮肤利什曼病的发病率有影响。本研究的目的是调查与健康个体相比，VDR基因多态性和/或血清维生素D水平与感染患者利什曼病之间的关联。在这项病例对照研究中，分别使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和电化学发光法，对50例感染[具体寄生虫名称未给出]的伊朗患者和健康对照者的VDR基因中的BsmI、FokI和Taq1多态性以及血清维生素D水平进行了研究。使用SPSS软件、卡方检验和方差分析对数据进行统计学分析。本研究结果表明，尽管与健康个体相比，患者中BsmI-BB、FokI-FF和TaqI-Tt的频率相对高于非BsmI-BB、非FokI-FF和非TaqI-Tt，但差异无统计学意义（P>0.05）。根据我们的研究结果，VDR多态性、25-羟基维生素D的血清浓度与[具体寄生虫名称未给出]感染易感性之间的关系仍不清楚，需要进一步深入研究。然而，为了更好地解释，有必要考虑诸如所检测样本的大小以及VDR的其他等位基因（包括ApaI）等因素。
