Cai Kunzhan, Jiao Yilai, Quan Quan, Hao Yulin, Liu Jie, Wu Lin
School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, 110001, China.
Shenyang National Laboratory for Materials Science, Institute of Metal Research, Chinese Academy of Sciences, Shenyang, 110016, China.
Bioact Mater. 2021 Apr 21;6(11):4073-4082. doi: 10.1016/j.bioactmat.2021.04.016. eCollection 2021 Nov.
Developing bioactive materials for bone implants to enhance bone healing and bone growth has for years been the focus of clinical research. Barium titanate (BT) is an electroactive material that can generate electrical signals in response to applied mechanical forces. In this study, a BT piezoelectric ceramic coating was synthesized on the surface of a TC4 titanium alloy, forming a BT/TC4 material, and low-intensity pulsed ultrasound (LIPUS) was then applied as a mechanical stimulus. The combined effects on the biological responses of MC3T3-E1 cells were investigated. Results of scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray diffraction showed that an uniform nanospheres -shaped BT coating was formed on TC4 substrate. Piezoelectric behaviors were observed using piezoelectric force microscopy with the piezoelectric coefficient d of 0.42 pC/N. Electrochemical measures indicated that LIPUS-stimulated BT/TC4 materials could produce a microcurrent of approximately 10 μA/cm. , the greatest osteogenesis (cell adhesion, proliferation, and osteogenic differentiation) was found in MC3T3-E1 cells when BT/TC4 was stimulated using LIPUS. Furthermore, the intracellular calcium ion concentration increased in these cells, possibly because opening of the L-type calcium ion channels was promoted and expression of the Ca1.2 protein was increased. Therefore, the piezoelectric BT/TC4 material with LIPUS loading synergistically promoted osteogenesis, rending it a potential treatment for early stage formation of reliable bone-implant contact.
开发用于骨植入物的生物活性材料以促进骨愈合和骨生长多年来一直是临床研究的重点。钛酸钡（BT）是一种电活性材料，可在施加机械力时产生电信号。在本研究中，在TC4钛合金表面合成了BT压电陶瓷涂层，形成BT/TC4材料，然后施加低强度脉冲超声（LIPUS）作为机械刺激。研究了其对MC3T3-E1细胞生物学反应的联合作用。扫描电子显微镜、能量色散X射线光谱和X射线衍射结果表明，在TC4基体上形成了均匀的纳米球形BT涂层。使用压电系数d为0.42 pC/N的压电显微镜观察到压电行为。电化学测量表明，LIPUS刺激的BT/TC4材料可产生约10 μA/cm的微电流。当使用LIPUS刺激BT/TC4时，在MC3T3-E1细胞中发现了最大的成骨作用（细胞粘附、增殖和成骨分化）。此外，这些细胞内的钙离子浓度增加，可能是因为L型钙离子通道的开放被促进且Ca1.2蛋白的表达增加。因此，加载LIPUS的压电BT/TC4材料协同促进成骨，使其成为早期形成可靠骨-植入物接触的潜在治疗方法。
