State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, P. R. China.
Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, P. R. China.
J Sep Sci. 2020 Jun;43(12):2459-2466. doi: 10.1002/jssc.201901242. Epub 2020 Apr 13.
Macleaya cordata (Willd) R. Br. is a medicinal plant. The most important bioactive compounds of M. cordata are alkaloids that have many biological activities including antifungal, anti-inflammatory, and antitumor. In this study, an ionic-liquid-modified high-speed counter-current chromatography method was established to obtain alkaloids from the fruits of M. cordata. The conditions of ionic-liquid-modified high-speed counter-current chromatography, including solvent systems, the content of ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate [C mim][BF ]), and the posttreatment of the ionic liquid, were investigated. Five alkaloids protopine, allocryptopine, sanguinarine, 8-O-demethylchelerythrine, and chelerythrine were separated from the extract of the fruits using a high speed counter-current chromatography with two-phase solvent system composed of dichloromethane/methanol/0.3 mol/L hydrochloric acid aqueous solution/[C mim][BF ] (4:2:2:0.015, v/v). Their purities were 96.33, 95.56, 97.94, 96.22, and 97.90%, respectively. The results indicated that a small amount of ionic liquids as modifier of the two-phase solvent system could shorten the separation time and improve the separation efficiency of the alkaloids from the fruits. The ionic-liquid-modified high-speed counter-current chromatography would provide a feasible way for highly effective separation of alkaloids from natural products.
头花蓼（Macleaya cordata (Willd) R. Br.）是一种药用植物。头花蓼最重要的生物活性化合物是生物碱，具有许多生物活性，包括抗真菌、抗炎和抗肿瘤。在本研究中，建立了一种离子液体改性高速逆流色谱法，从头花蓼的果实中获得生物碱。研究了离子液体改性高速逆流色谱的条件，包括溶剂系统、离子液体（1-丁基-3-甲基咪唑四氟硼酸盐 [C mim][BF ]）的含量和离子液体的后处理。使用两相溶剂系统（二氯甲烷/甲醇/0.3 mol/L 盐酸水溶液/[C mim][BF ]（4:2:2:0.015，v/v））通过高速逆流色谱从果实提取物中分离出 5 种生物碱：普罗托品、Allocryptopine、血根碱、8-O-去甲白屈菜红碱和白屈菜红碱。它们的纯度分别为 96.33%、95.56%、97.94%、96.22%和 97.90%。结果表明，少量离子液体作为两相溶剂系统的改性剂可以缩短分离时间，提高生物碱的分离效率。离子液体改性高速逆流色谱为从天然产物中高效分离生物碱提供了一种可行的方法。
