Dong Zhen, Wang Yu-Hong, Tang Zhao-Shan, Li Chang-Hong, Jiang Tao, Yang Zi-Hui, Zeng Jian-Guo
College of Veterinary Medicine, Hunan Agricultural University, Changsha, China.
Key Laboratory of Chinese Veterinary Medicine in Hunan Province, Hunan Agricultural University, Changsha, China.
Front Vet Sci. 2022 Jul 5;9:935201. doi: 10.3389/fvets.2022.935201. eCollection 2022.
(Willd). R. Br. is a Chinese medicinal plant commonly used externally to treat inflammatory-related diseases such as arthritis, sores, and carbuncles. This study aimed to evaluate the anti-inflammatory activity of protopine total alkaloids (MPTAs) in (Willd.) R. Br. tests in rats with acute inflammation showed that MPTA (2.54 and 5.08 mg/kg) showed significant anti-inflammatory activity 6 h after carrageenan injection. Similarly, MPTA (3.67 and 7.33 mg/kg) showed significant anti-inflammatory activity in the mouse ear swelling test. In addition, the potential mechanisms of the anti-inflammatory effects of MPTA were explored based on network pharmacology and molecular docking. The two main active components of MPTA, protopine and allocryptopine, were identified, and the potential targets and signaling pathways of MPTA's anti-inflammatory effects were initially revealed using tools and databases (such as SwissTargetPrediction, GeneCards, and STRING) combined with molecular docking results. This study provides the basis for the application of MPTA as an anti-inflammatory agent.
威灵仙是一种常用的中药，通常外用治疗与炎症相关的疾病，如关节炎、疮疡和痈肿。本研究旨在评估威灵仙中原托品总生物碱（MPTAs）的抗炎活性。在大鼠急性炎症试验中表明，角叉菜胶注射后6小时，MPTA（2.54和5.08毫克/千克）显示出显著的抗炎活性。同样，MPTA（3.67和7.33毫克/千克）在小鼠耳肿胀试验中显示出显著的抗炎活性。此外，基于网络药理学和分子对接探索了MPTA抗炎作用的潜在机制。确定了MPTA的两种主要活性成分原托品和别隐品碱，并结合分子对接结果，使用工具和数据库（如SwissTargetPrediction、GeneCards和STRING）初步揭示了MPTA抗炎作用的潜在靶点和信号通路。本研究为MPTA作为抗炎剂的应用提供了依据。
