Institute of TCM and Natural Products, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, P.R. China.
Food Funct. 2020 Mar 1;11(3):2749-2762. doi: 10.1039/c9fo01902b. Epub 2020 Mar 16.
Ultraviolet B (UVB) irradiation-induced DNA damage, oxidative stress, inflammatory processes, and skin pigmentation cause pigmented spots, wrinkles, inflammation, and accelerated skin aging and cancer. Maqui berry (Aristotelia chilensis) is a natural antioxidant, anticancer, and anti-inflammatory food. We investigated the photoprotective properties of the ethyl acetate fraction of maqui berry ethanol extract (MEE) in vitro and in vivo. Spectrophotometric measurements revealed dominant extinction profile of MEE in the UVB range. MEE clearly reversed the DNA damage induced by UVB irradiation in HaCaT cells by upregulating endogenous cellular enzymatic and non-enzymatic antioxidant systems containing superoxide dismutase, catalase, and glutathione and reducing the production of nitric oxide. Moreover, MEE treatment enhanced the antioxidant ability and weakened lipid peroxidation in BALB/c mice exposed to UVB radiation. It also down-regulated interleukin (IL)-6 and tumor necrosis factor-α levels and up-regulated IL-4 levels. Moreover, MEE inhibited the UVB-triggered activation of ERK and p38 MAPK. These data suggest that MEE is an effective agent against UVB-induced photodamage.
紫外线 B（UVB）照射引起的 DNA 损伤、氧化应激、炎症过程和皮肤色素沉着导致色素斑、皱纹、炎症以及加速皮肤衰老和癌症。智利酒果（Aristotelia chilensis）是一种天然抗氧化剂、抗癌和抗炎食品。我们研究了智利酒果乙醇提取物的乙酸乙酯部分（MEE）的体外和体内光保护特性。分光光度测量显示 MEE 在 UVB 范围内具有占主导地位的消光特性。MEE 通过上调超氧化物歧化酶、过氧化氢酶和谷胱甘肽等内源性细胞酶和非酶抗氧化系统，并减少一氧化氮的产生，明显逆转了 UVB 照射诱导的 HaCaT 细胞中的 DNA 损伤。此外，MEE 处理增强了暴露于 UVB 辐射的 BALB/c 小鼠的抗氧化能力并减弱了脂质过氧化。它还下调了白细胞介素（IL）-6 和肿瘤坏死因子-α水平，并上调了 IL-4 水平。此外，MEE 抑制了 UVB 触发的 ERK 和 p38 MAPK 的激活。这些数据表明，MEE 是一种对抗 UVB 诱导的光损伤的有效药物。
