State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, PR China.
Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2719-2725. doi: 10.1099/ijsem.0.004097. Epub 2020 Mar 16.
Four strains assigned the names FT13W, FT14W, FT58W and FT68W were isolated from a subtropical stream in PR China. All the strains were Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and motile with flagella. Comparisons based on 16S rRNA gene sequences showed that strains FT13W, FT14W, FT58W and FT68W belonged to genus and shared 16S rRNA gene similarities in the range of 98.8-99.7 % with DSM 1522, DSM 9628 and ' JA-1', respectively. The calculated pairwise average nucleotide identity (ANI) values among the genomes of above seven strains were in the range of 79.0-92.2 %, except that the ANI value was 96.8 % between strain FT13W and FT14W. The respiratory quinone of strains FT13W, FT14W, FT58W and FT68W was determined to be Q-8. The major fatty acids were C ω7, C, C ω7 and C. The polar lipids included phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid. The genome sizes of strains FT13W, FT14W, FT58W and FT68W were 6.45, 6.38, 5.73 and 6.37 Mbp with G+C contents of 63.4, 63.7, 61.6 and 63.1 mol%, respectively. Combining phenotypic, biochemical, genotypic and ANI data, strain FT13W and FT14W should belong to the same species. The four strains were considered to represent three novel species within genus , for which the names sp. nov. (type strain FT13W=GDMCC 1.1638=KACC 21319), sp. nov. (FT58W=GDMCC 1.1676=KACC 21468) and sp. nov. (FT68W=GDMCC 1.1677=KACC 21469) are proposed.
从中国亚热带的一条溪流中分离到了四株菌,分别命名为 FT13W、FT14W、FT58W 和 FT68W。这四株菌均为革兰氏阴性、过氧化氢酶和氧化酶阳性、杆状、有鞭毛、可运动。基于 16S rRNA 基因序列的比较表明,菌株 FT13W、FT14W、FT58W 和 FT68W 属于 属,与 DSM 1522、DSM 9628 和'JA-1'的 16S rRNA 基因相似度分别为 98.8-99.7%。上述 7 株菌的基因组之间的平均核苷酸同一性(ANI)值计算值在 79.0-92.2%之间,除了菌株 FT13W 和 FT14W 的 ANI 值为 96.8%。菌株 FT13W、FT14W、FT58W 和 FT68W 的呼吸醌为 Q-8。主要脂肪酸为 C 17:0、C 16:0、C 17:1ω7c 和 C 16:1ω7c。极性脂包括磷脂酰乙醇胺、磷脂酰甘油和一种未鉴定的磷脂。菌株 FT13W、FT14W、FT58W 和 FT68W 的基因组大小分别为 6.45、6.38、5.73 和 6.37 Mbp,G+C 含量分别为 63.4、63.7、61.6 和 63.1 mol%。结合表型、生化、基因型和 ANI 数据,菌株 FT13W 和 FT14W 应属于同一物种。这四株菌被认为代表了 属内的三个新种,分别命名为 sp. nov.(模式菌株 FT13W=GDMCC 1.1638=KACC 21319)、 sp. nov.(FT58W=GDMCC 1.1676=KACC 21468)和 sp. nov.(FT68W=GDMCC 1.1677=KACC 21469)。