Hospital Universitario Miguel Servet, Instituto de Investigación Sanitaria Aragón (IIS Aragón), CIBERCV, Universidad de Zaragoza, Zaragoza 50009, Spain.
Growth, Exercise, Nutrition and Development (GENUD) Research Group, Instituto Agroalimentario de Aragón (IA2), Universidad de Zaragoza, Instituto de Investigación Sanitaria de Aragón (IIS Aragón), Zaragoza 50009, Spain.
Cells. 2020 Mar 12;9(3):693. doi: 10.3390/cells9030693.
The global prevalence of type 2 diabetes (T2D) is increasing rapidly, especially in low- and middle-income countries and has a high number of associated comorbidities. Plasmatic concentrations of branched chain amino acids (BCAA) and retinol-binding protein 4 (RBP4) have been shown to be elevated in T2D subjects in cross-sectional studies. However, the effect of lifestyle community-based interventions on BCAA and RBP4 concentrations has not yet been analyzed.
The Feel4Diabetes study is a school and community-based intervention that identified 360 European families with a high risk of developing T2D according to the FINDRISC questionnaire. Families were randomized in control and intervention groups were followed-up from 2016 to 2018. In the Spanish families, the concentration of BCAA and RBP4 was determined in 266 subjects (115 control and 151 intervention group) that attended the three time-point assessments by colorimetric and ELISA reaction, respectively.
Baseline BCAA levels showed positive correlations with the FINDRISC score and glucose impairment (baseline glucose, insulin, and glycated hemoglobin), body mass index, and body weight. The participants receiving the community-based intervention showed a significant decrease in glycated hemoglobin and BCAA levels compared to the control group ( = 0.011 and < 0.001, respectively). However, baseline RBP4 did not show significant correlations with anthropometric and glycemic parameters, and no significant change was observed in anthropometric parameters and RBP4 concentrations throughout the follow-up.
A community-based intervention on lifestyle led to a significant reduction in BCAA levels regardless of weight loss. These findings suggest that this interventional approach could be promising in T2D prevention.
2 型糖尿病（T2D）在全球的患病率正在迅速上升，尤其是在中低收入国家，且伴有大量合并症。在横断面研究中已表明，T2D 患者的支链氨基酸（BCAA）和视黄醇结合蛋白 4（RBP4）的血浆浓度升高。然而，基于生活方式的社区干预对 BCAA 和 RBP4 浓度的影响尚未进行分析。
Feel4Diabetes 研究是一项基于学校和社区的干预研究，根据 FINDRISC 问卷确定了 360 个有发展为 T2D 高风险的欧洲家庭。家庭被随机分为对照组和干预组，随访时间从 2016 年到 2018 年。在西班牙家庭中，通过比色法和 ELISA 反应分别测定了 266 名受试者（115 名对照组和 151 名干预组）的 BCAA 和 RBP4 浓度。
基线时 BCAA 水平与 FINDRISC 评分以及葡萄糖损伤（基线血糖、胰岛素和糖化血红蛋白）、体重指数和体重呈正相关。与对照组相比，接受社区干预的参与者的糖化血红蛋白和 BCAA 水平显著降低（ = 0.011 和 < 0.001）。然而，基线 RBP4 与人体测量和血糖参数无显著相关性，且在整个随访过程中人体测量参数和 RBP4 浓度无显著变化。
无论体重减轻与否，基于生活方式的社区干预都能显著降低 BCAA 水平。这些发现表明，这种干预方法在 T2D 预防方面可能很有前景。
