Comparative Survival and the Cold-Induced Gene Expression of Pathogenic and Nonpathogenic from Tropical Eastern Oysters during Cold Storage.

Expression of the regulatory stress gene controls the transcription of genes, which are involved in survival and adaptation to low temperatures. The purpose of this study was to assess the growth kinetics of naturally occurring . in shellstock oysters and in vitro and the cold-shock-induced expression of the and gene response in vitro during postharvest refrigeration. Naturally contaminated eastern oysters () and pathogenic () and nonpathogenic () isolates were stored at 7 ± 1 °C for 168 h and 216 h, respectively. The regulatory stress () and cold-shock () gene expressions were determined by reverse transcription PCR. At 24 h, the () strain grew faster ( < 0.05) than the () strain in oysters (λ = 0.33, 0.39, respectively) and in vitro (λ = 0.89, 37.65, respectively), indicating a better adaptation to cold shock for the () strain in live oysters and in vitro. At 24 h, the (-) strain and gene expressions were upregulated by 1.9 and 2.3-fold, respectively, but the ( strain and gene expressions were repressed and upregulated by -0.024 and 1.9-fold, respectively. The . strains that were isolated from tropical oysters have adaptive expression changes to survive and grow at 7 °C, according to their virulence.