Fu Rong, Ren Tuanhui, Li Wangyu, Liang Jiaying, Mo Guodong, Luo Wen, He Danlin, Liang Shaodong, Zhang Xiquan
Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, China.
Animals (Basel). 2020 Mar 12;10(3):475. doi: 10.3390/ani10030475.
Golgin subfamily B member 1 () gene encodes the coat protein 1 vesicle inhibiting factor, giantin. Previous study showed that mutations of the gene are associated with dozens of human developmental disorders and diseases. However, the biological function of gene in chicken is still unclear. In this study, we detected a novel 65-bp insertion/deletion (indel) polymorphism in the chicken intron 5. Association of this indel with chicken growth and carcass traits was analyzed in a yellow chicken population. Results showed that this 65-bp indel was significantly associated with chicken body weight ( < 0.05), highly significantly associated with neck weight, abdominal fat weight, abdominal fat percentage and the yellow index b of breast ( < 0.01). Analysis of genetic parameters indicated that "" was the predominant allele. Except for the yellow index b of breast, genotype individuals had the best growth characteristics, by comparison with the genotype and genotype individuals. Moreover, the mRNA expression of was detected in the liver tissue of chicken with different genotypes, where the genotype displayed high expression levels. These findings hinted that the 65-bp indel in could be assigned to a molecular marker in chicken breeding and enhance production in the chicken industry.
高尔基体蛋白亚家族B成员1（）基因编码衣被蛋白1囊泡抑制因子巨蛋白。先前的研究表明，该基因的突变与数十种人类发育障碍和疾病相关。然而，该基因在鸡中的生物学功能仍不清楚。在本研究中，我们在鸡的第5内含子中检测到一种新的65 bp插入/缺失（indel）多态性。在一个黄羽鸡群体中分析了这种indel与鸡生长和胴体性状的关联。结果表明，这种65 bp indel与鸡体重显著相关（<0.05），与颈部重量、腹部脂肪重量、腹部脂肪百分比和胸肌黄度指数b高度显著相关（<0.01）。遗传参数分析表明，“”是优势等位基因。与基因型和基因型个体相比，除胸肌黄度指数b外，基因型个体具有最佳的生长特性。此外，在不同基因型鸡的肝脏组织中检测到的mRNA表达，其中基因型表达水平较高。这些发现表明，中的65 bp indel可作为鸡育种中的分子标记，提高养鸡业的产量。
