Department of Gastroenterology and Hepatology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China.
Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.
BMC Gastroenterol. 2020 Mar 16;20(1):73. doi: 10.1186/s12876-020-01212-3.
Short-chain fatty acids (SCFAs) alteration have been reported in irritable bowel syndrome (IBS), but the results are conflicting. Our study aims to explore the alteration of SCFAs in patients with diarrhea-predominant IBS (IBS-D) and their potential role in the occurrence and development of IBS.
We recruited patients with IBS-D defined by Rome IV criteria and age-and-gender matched healthy controls (HCs). A headspace solid-phase microextraction gas chromatography-mass spectrometric (HS-SPME-GC-MS) method was developed for the analysis of acetic, propionic and butyric acid in feces and serum.
Compared with HCs, the levels of the serum propionate (2.957 ± 0.157 vs 2.843 ± 0.098 mmol/L, P = 0.012) and butyrate (2.798 ± 0.126 vs 2.697 ± 0.077 mmol/L, P = 0.012) were significantly higher in IBS-D group. No significant differences were found among two groups with regard to the concentration of fecal acetate (4.953 ± 1.065 vs 4.774 ± 1.465 mg/g, P = 0.679), propionate (6.342 ± 1.005 vs 6.282 ± 1.077 mg/g, P = 0.868) and butyrate (2.984 ± 0.512 vs 3.071 ± 0.447 mg/g, P = 0.607).
Metabolites of gut microbiota, the propionic and butyric acid, are increased in patients with IBS-D in serum but not in feces. It suggests that propionic and butyric acid might be associated with the occurrence and development of IBS.
短链脂肪酸（SCFAs）在肠易激综合征（IBS）中发生改变，但结果存在争议。本研究旨在探讨腹泻型肠易激综合征（IBS-D）患者中 SCFAs 的变化及其在 IBS 发生和发展中的潜在作用。
我们招募了符合 Rome IV 标准的 IBS-D 患者和年龄、性别匹配的健康对照者（HCs）。采用顶空固相微萃取气相色谱-质谱联用（HS-SPME-GC-MS）法分析粪便和血清中乙酸、丙酸和丁酸的水平。
与 HCs 相比，IBS-D 组血清丙酸盐（2.957±0.157 vs 2.843±0.098mmol/L，P=0.012）和丁酸盐（2.798±0.126 vs 2.697±0.077mmol/L，P=0.012）水平显著升高。两组粪便中乙酸（4.953±1.065 vs 4.774±1.465mg/g，P=0.679）、丙酸（6.342±1.005 vs 6.282±1.077mg/g，P=0.868）和丁酸（2.984±0.512 vs 3.071±0.447mg/g，P=0.607）浓度无显著差异。
肠道微生物代谢物丙酸盐和丁酸盐在 IBS-D 患者血清中增加，但在粪便中没有增加。这表明丙酸盐和丁酸盐可能与 IBS 的发生和发展有关。
