秦川牛脂肪组织相关 lncRNA 的转录组分析。

Transcriptome profiling of lncRNA related to fat tissues of Qinchuan cattle.

机构信息

Shaanxi Key Laboratory of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China.

Shaanxi Key Laboratory of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530004, Guangxi, China.

出版信息

Gene. 2020 Jun 5;742:144587. doi: 10.1016/j.gene.2020.144587. Epub 2020 Mar 13.

DOI:10.1016/j.gene.2020.144587

PMID:32179170

Abstract

Qinchuan cattle is one of the five yellow cattle breeds in China with good performance of meat. The proliferation and differentiation level of muscle and fat are closely related to the growth and development of the organism and are the key factors affecting the quality of meat. In order to study the effect of lncRNA on the fat tissues of Qinchuan cattle, six calf and adult bovine adipose tissues were selected for high-throughput sequencing. We obtained 3,716 lncRNA candidates from calves and adult cattle fat samples, among them 789 lncRNA were annotated and 2,927 lncRNA were novel lncRNA. A number of lncRNAs were highly abundant, and 119 lncRNA were differentially expressed between two developmental stages. We further validated several differentially expressed lncRNAs using qPCR, and the results were consistent with the sequencing data. Therefore, we conclude that lncRNA may play an important role in adipose tissue in different age groups of cattle.

摘要

秦川牛是中国五大黄牛品种之一，具有良好的肉用性能。肌肉和脂肪的增殖和分化水平与机体的生长发育密切相关，是影响肉质的关键因素。为了研究 lncRNA 对秦川牛脂肪组织的影响，本研究从犊牛和成年牛脂肪组织中选取了 6 个样本进行高通量测序。我们从犊牛和成年牛脂肪样本中获得了 3716 个 lncRNA 候选物，其中 789 个 lncRNA 被注释，2927 个 lncRNA 是新的 lncRNA。许多 lncRNA 高度丰富，在两个发育阶段之间有 119 个 lncRNA 差异表达。我们进一步使用 qPCR 对几个差异表达的 lncRNA 进行了验证，结果与测序数据一致。因此，我们得出结论，lncRNA 可能在不同年龄组牛的脂肪组织中发挥重要作用。