Department of Chemistry and Chemical Biology, UNM Comprehensive Cancer Center, University of New Mexico, 300 Terrace St. NE, Albuquerque, NM 87131, USA.
Department of Medicinal Chemistry, College of Pharmacy, UF Health Science Center, UF Health Cancer Center University of Florida, 1345 Center Dr., Gainesville, FL 32610, USA.
Chembiochem. 2020 Aug 3;21(15):2196-2204. doi: 10.1002/cbic.202000056. Epub 2020 Apr 16.
Fluorophores experience altered emission lifetimes when incorporated into and liberated from macromolecules or molecular aggregates; this trend suggests the potential for a fluorescent, responsive probe capable of undergoing self-assembly and aggregation and consequently altering the lifetime of its fluorescent moiety to provide contrast between the active and inactive probes. We developed a cyanobenzothioazole-fluorescein conjugate (1), and spectroscopically examined the lifetime changes caused by its reduction-induced aggregation in vitro. A decrease in lifetime was observed for compound 1 in a buffered system activated by the biological reducing agent glutathione, thus suggesting a possible approach for designing responsive self-aggregating lifetime imaging probes.
荧光团在被整合到生物大分子或分子聚集体中以及从其中释放出来时，其发射寿命会发生改变；这种趋势表明，有可能开发出一种荧光响应探针，它能够进行自组装和聚集，从而改变其荧光部分的寿命，以提供活性和非活性探针之间的对比度。我们开发了一种氰基苯并噻唑-荧光素缀合物（1），并在体外研究了其还原诱导聚集引起的寿命变化。在由生物还原剂谷胱甘肽激活的缓冲体系中，观察到化合物 1 的寿命降低，因此这为设计响应性自聚集寿命成像探针提供了一种可能的方法。
