Brochier B, Thomas I, Iokem A, Ginter A, Kalpers J, Paquot A, Costy F, Pastoret P P
Department of Virology, Faculty of Veterinary Medicine, University of Liège, Brussels, Belgium.
Vet Rec. 1988 Dec 10;123(24):618-21.
Campaigns of fox vaccination against rabies were carried out in Belgium in September 1986 and June and September 1987. The SAD B19 attenuated strain of rabies virus was inserted into baits which were distributed over an area of 2100 km2 at a density of 11 baits/km2. As recommended by the World Health Organisation, the efficacy and the innocuity of the method were controlled in the field and in the laboratory. Samples of blood and brain and jaw were taken from foxes which were shot or found dead in the vaccination area, for the diagnosis of rabies, the titration of antirabies antibody and the detection of tetracycline marker. In rabid animals, the virus strain was characterised by immunofluorescence using monoclonal antibodies. In September 1987, the uptake of the baits had reached 72 per cent by 14 days after distribution. Several wild species competed with foxes in taking the baits. After the last campaign, tetracycline was found in 65 per cent of the healthy foxes collected and rabies virus neutralising antibodies were detected in 77 per cent of them. In 1987, the incidence of rabies decreased markedly in the vaccination area compared with the untreated areas. No vaccine virus was isolated either from rabid animals or from 228 small mammals trapped in the vaccination area.
1986年9月以及1987年6月和9月，比利时开展了针对狐狸的狂犬病疫苗接种行动。狂犬病病毒的SAD B19减毒株被植入诱饵中，这些诱饵以每平方公里11个的密度分布在2100平方公里的区域。按照世界卫生组织的建议，该方法的有效性和安全性在野外和实验室进行了监测。从在疫苗接种区域被射杀或发现死亡的狐狸身上采集血液、大脑和颌骨样本，用于狂犬病诊断、抗狂犬病抗体滴定以及四环素标记物检测。对于患狂犬病的动物，使用单克隆抗体通过免疫荧光对病毒株进行鉴定。1987年9月，在诱饵分发14天后，其摄取率达到了72%。几种野生动物与狐狸争抢诱饵。在最后一次行动之后，在采集的健康狐狸中，65%检测到含有四环素，77%检测到有狂犬病病毒中和抗体。1987年，与未处理区域相比，疫苗接种区域的狂犬病发病率显著下降。在患狂犬病的动物身上以及在疫苗接种区域捕获的228只小型哺乳动物身上均未分离出疫苗病毒。
